Protein Info for Psest_3016 in Pseudomonas stutzeri RCH2

Annotation: TonB-dependent vitamin B12 receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR01779: TonB-dependent vitamin B12 receptor" amino acids 15 to 616 (602 residues), 789.9 bits, see alignment E=8.8e-242 PF07715: Plug" amino acids 47 to 152 (106 residues), 107.7 bits, see alignment E=4.4e-35 PF00593: TonB_dep_Rec" amino acids 175 to 590 (416 residues), 164.6 bits, see alignment E=7.5e-52

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 94% identity to psa:PST_1289)

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQ74 at UniProt or InterPro

Protein Sequence (617 amino acids)

>Psest_3016 TonB-dependent vitamin B12 receptor (Pseudomonas stutzeri RCH2)
MTCKPRFKPAAYVLLGSFPLSIGQLAAAPLDLQQQVVTATRTAQTAQQSLAAVTVIDRER
IERSQASSLPELLKQVPGVSLANNGGPGKSTSLFMRGTESDHVLVMIDGIKIGSVSGGGA
ALQDLPLELIERIEVVRGPRSSLYGSEAIGGVIQIFTRKGQGQGVKPFFSAGYGTHDTYT
GSAGVSGGDGRGWYSLGVSGSDTDGINVKSADTSGYERDADGYRNLSATLSAGYRFDNGL
ELDANLLQAKSHNDYDSVNRARTSGFGANADGESRVFGTRARFSPLEPWLVTLQAGRSED
KSDAYQDGQFYSRFDSRRDTLSWQNDIELAAGHTLILGADHQRDEVNGTTVYAEDSRDND
GYFAQYLGEVGRHDWQLSLRRDDNEQFGQHDTGNIGYGYALTDWLRGTISYGTAFKAPTF
NELYFPDYGNPELDAETSESLEVGLAGQHGWGHWAVNAYRTVVDDLIAYDASISAPANVQ
EARIRGVELVLGSQLLGWDWNANYSLLEPENRSAGANRGNELARRAKQLFNLDLDRRLGA
FSVGASLHAEGQRYDDLANTRELSGYATLDLRGEYRITPEWRLQARVANLLDADYETAEG
YNQPGQAAYLTVRYQAL