Protein Info for Psest_3008 in Pseudomonas stutzeri RCH2

Annotation: cobyric acid synthase CobQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 PF13500: AAA_26" amino acids 3 to 228 (226 residues), 60.3 bits, see alignment E=3.6e-20 TIGR00313: cobyric acid synthase CobQ" amino acids 4 to 473 (470 residues), 488.8 bits, see alignment E=9.1e-151 PF01656: CbiA" amino acids 4 to 229 (226 residues), 82.6 bits, see alignment E=3.5e-27 PF07685: GATase_3" amino acids 250 to 433 (184 residues), 197.8 bits, see alignment E=2.2e-62

Best Hits

Swiss-Prot: 92% identical to COBQ_PSEU5: Cobyric acid synthase (cobQ) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K02232, adenosylcobyric acid synthase [EC: 6.3.5.10] (inferred from 92% identity to psa:PST_1297)

MetaCyc: 50% identical to CobQ (Pseudomonas denitrificans (nom. rej.))
Adenosylcobyric acid synthase (glutamine-hydrolyzing). [EC: 6.3.5.10]

Predicted SEED Role

"Cobyric acid synthase (EC 6.3.5.10)" (EC 6.3.5.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GP04 at UniProt or InterPro

Protein Sequence (485 amino acids)

>Psest_3008 cobyric acid synthase CobQ (Pseudomonas stutzeri RCH2)
MTTLMVQGTTSDAGKSTLVTALCRWLARQGVSVVPFKPQNMALNSAVTADGGEIGRAQAV
QAQAANLAPHTDMNPVLLKPNSDIGAQVIIHGRAVTSMDAAAYHDYKRVAMQAVLDSHRR
LSAGYRVVMVEGAGSPAEINLRAGDIANMGFAEAVECPVILIADIDKGGVFAHLVGTLAL
LSESEQARVQGFVINRFRGDIALLQPGLDWLEQRTGKPVLGVLPYLMDFHLEAEDAIDVR
QSGKTGEALKVVVPVLPRISNHTDFDPLRLHPQVDLQFVGPGHPIPAADLIILPGSKSVR
ADLTWLRANGWETAIRKHLRYGGKLLGICGGLQMLGTRIADPLGLEGAAGESAGLGLLDI
ATVLEAEKQLRNVTGRLLPGGACVTGYEIHAGVSSGPALEQPAVRLDDGRCDGAISADGQ
ILGTYLHGLFEGPEACAALLRWAGLDAVHTVDYHALRERDIERLADLVEMHLDTPRLRAL
CGLGG