Protein Info for PGA1_c29910 in Phaeobacter inhibens DSM 17395

Annotation: lead, cadmium, zinc and mercury-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 transmembrane" amino acids 111 to 129 (19 residues), see Phobius details amino acids 135 to 153 (19 residues), see Phobius details amino acids 161 to 179 (19 residues), see Phobius details amino acids 185 to 202 (18 residues), see Phobius details amino acids 335 to 356 (22 residues), see Phobius details amino acids 363 to 390 (28 residues), see Phobius details amino acids 671 to 688 (18 residues), see Phobius details amino acids 693 to 715 (23 residues), see Phobius details PF00403: HMA" amino acids 12 to 71 (60 residues), 40.9 bits, see alignment 4.4e-14 TIGR01511: copper-translocating P-type ATPase" amino acids 164 to 714 (551 residues), 427.1 bits, see alignment E=2.5e-131 TIGR01512: cadmium-translocating P-type ATPase" amino acids 165 to 471 (307 residues), 323.9 bits, see alignment E=3.5e-100 TIGR01525: heavy metal translocating P-type ATPase" amino acids 165 to 714 (550 residues), 607.2 bits, see alignment E=7.2e-186 TIGR01494: HAD ATPase, P-type, family IC" amino acids 188 to 691 (504 residues), 269.5 bits, see alignment E=9e-84 PF00122: E1-E2_ATPase" amino acids 216 to 396 (181 residues), 184.4 bits, see alignment E=2.9e-58 PF00702: Hydrolase" amino acids 414 to 625 (212 residues), 124.2 bits, see alignment E=1.8e-39 PF08282: Hydrolase_3" amino acids 595 to 651 (57 residues), 26.7 bits, see alignment 8.8e-10

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 66% identity to pde:Pden_2344)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DU90 at UniProt or InterPro

Protein Sequence (726 amino acids)

>PGA1_c29910 lead, cadmium, zinc and mercury-transporting ATPase (Phaeobacter inhibens DSM 17395)
MAKDTGEQLEWRVTGMDCGSCATKLRGAVERLPGVSGVEVAMMAERLRLDLDPQEGSVIA
VEKAVRKLGFDLMARGDGAGVDRNPCCDTPPAGASTATEAPAWYRSGKGQLLLGTGLLLA
LAWGINLLASPTVGLWAFMLATLIGVAPIARRAFAMLRAGMPFTIEMLMSIAAIGALFIG
AAEEAALVVFLFAVGEMLEGLASNKARDGIRALADLVPKTALVERGDVLEDVAADSLREG
QIVVVRPGDRVPADGAVIDGVSGVDESPVTGESVPRLKEPGSEVFAGSINAEAVLRVRVT
RAAADNTISRIIRLVEEAESARAPTERFIDRFSRVYMPIIVGVALLVALVPPLGFGLDWN
TWIYRALALLLIGCPCALVISVPAAIASALSAGARHGLLLKGGAVIEAAAGTTHVAFDKT
GTLTRGRPQVTDIVVHHGSEDKLLELAAAVERESSHPLAEAICARAADSGVDSPLVQEAR
AVLGKGASAKVGSLVITVGSPRFASETGVMTETTIAQTAKLEAQGKTVVVLFSDEVLYGL
IALRDEPREDAADAVQKLKRMGINATMLTGDNARTAEAIAGQLGLDHRAELMPEDKVTAL
QDLTRSAQVMMVGDGINDAPALATAQVGVAMGSGTDVALETADAAILRNRVSDVVGVIRL
SRATLTNIRQNVAVALGLKGVFLVTSVLGMTGLWIAILADTGATVLVTLNALRLLSFKPS
LTSSDN