Protein Info for PGA1_c29830 in Phaeobacter inhibens DSM 17395

Annotation: 3-isopropylmalate dehydrogenase LeuB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00180: Iso_dh
  • amino acids 6 to 359 (354 residues), 449.3 bits, E=5.4e-139
• TIGR00169: 3-isopropylmalate dehydrogenase
  • amino acids 6 to 358 (353 residues), 485.6 bits, E=4.1e-150

Best Hits

Swiss-Prot: 92% identical to LEU3_RUEPO: 3-isopropylmalate dehydrogenase (leuB) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 92% identity to sil:SPO0210)

MetaCyc: 52% identical to isopropylmalate dehydrogenase 3 (Arabidopsis thaliana col)
3-isopropylmalate dehydrogenase. [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.85

Sequence Analysis Tools

See I7E4C4 at UniProt or InterPro

Protein Sequence (367 amino acids)

>PGA1_c29830 3-isopropylmalate dehydrogenase LeuB (Phaeobacter inhibens DSM 17395)
MSNPSILILPGDGIGPEVMAEVRKVISWYGEKRDLQFDVSEDLVGGAAYDVHGKPLADET
MEKAQEVDAVLLGAVGGPKYDDLDFSVKPERGLLRLRKEMDLYSNLRPAQCFDALADFSS
LKKDIVAGLDIMIVRELTSGVYFGEPRGIFEEGNERVGINTQRYTESEIERAARSAFELA
MRRNKKLCSMEKANVMESGILWREVVTRVAADYPEVELSHMYADNGAMQLVRAPKQFDVI
LTDNLFGDILSDCAAMLTGSLGMLPSASLGAPMANGRPKALYEPVHGSAPDIAGQGKANP
IACILSFAMALRYSFDQGSEADRLETAVEKVLADGHRTGDLLGEEGVSPISTSEMGDVII
AALDDSL