Protein Info for Psest_2989 in Pseudomonas stutzeri RCH2

Annotation: alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00344: alanine--tRNA ligase
  • amino acids 5 to 853 (849 residues), 1062.1 bits, E=0
• PF01411: tRNA-synt_2c
  • amino acids 5 to 552 (548 residues), 751.9 bits, E=6.4e-230
• PF07973: tRNA_SAD
  • amino acids 651 to 695 (45 residues), 63.6 bits, 2.1e-21
• PF02272: DHHA1
  • amino acids 734 to 868 (135 residues), 102.1 bits, E=5.3e-33

Best Hits

Swiss-Prot: 66% identical to SYA_ECOL5: Alanine--tRNA ligase (alaS) from Escherichia coli O6:K15:H31 (strain 536 / UPEC)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 84% identity to avn:Avin_34460)

MetaCyc: 66% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• superpathway of chorismate metabolism (44/59 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

See L0GL83 at UniProt or InterPro

Protein Sequence (874 amino acids)

>Psest_2989 alanine--tRNA ligase (Pseudomonas stutzeri RCH2)
MKSAEIREAFLRFFEEKGHTRVASSSLIPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRA
TTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWEFLTSDKWLN
LPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWAMGDTGPCGPC
TEIFFDHGEHIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVLHPLPAPSVDTGMGLE
RISAVLQHVNSNYEIDLFQSLLNAAADAIGCANEGQASLKVVADHIRSCGFLISDGVTPS
NEGRGYVLRRIVRRACRHGNKLGAKGSFFHRIVAALVAEMGEAFPGLKQQQAHIERVLKN
EEEQFAKTLEQGLKILEQDLAGLAGSVIPGDVVFKLYDTYGFPVDLTGDIARERNLTLDE
EGFEREMQAQRERARSASAFGMDYNSLVKVEGETRFIGYQGTSGTGKVLALFKEGMVVDS
LSAGEEGVVVLDQTPFYAESGGQIGDCGYLEAQGLRFDVRDTSKAGSAFLHHGIVDSGTL
RVGTGVQATVDATVRQATALNHSATHLLHAALREILGEHVSQKGSLVDSQRLRFDFSHFE
AIKPEQLRALEDRVNAEIRRNSAVEIEETDIDTAKAKGAMALFGEKYGDSVRVLTMGGGF
SVELCGGTHVNRTGDIGLFKITSEGGVAAGVRRIEAVTGAPALAYLNAAEEQLKEAANLL
KGSRDNLLDKLGGILERNRQLEKELEQLKAKAASAAGNDLAGSAVEVKGVRVLAARQDGL
DGKALLALVDQLKNKLGSAVILLGGVQDDKVVLVAGVTQDLTSRLKAGDLMKQAAAAVGG
KGGGRPDMAQGGGSEPARLDDALALALSFAEQGL