Protein Info for Psest_2980 in Pseudomonas stutzeri RCH2

Annotation: oxaloacetate decarboxylase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 PF00682: HMGL-like" amino acids 10 to 271 (262 residues), 66.8 bits, see alignment E=5.3e-22 TIGR01108: oxaloacetate decarboxylase alpha subunit" amino acids 10 to 588 (579 residues), 884.6 bits, see alignment E=1.5e-270 PF02436: PYC_OADA" amino acids 295 to 490 (196 residues), 199.8 bits, see alignment E=1.1e-62 PF00364: Biotin_lipoyl" amino acids 530 to 591 (62 residues), 61.5 bits, see alignment E=1.4e-20

Best Hits

Swiss-Prot: 67% identical to DCOA_KLEPN: Oxaloacetate decarboxylase alpha chain (oadA) from Klebsiella pneumoniae

KEGG orthology group: K01571, oxaloacetate decarboxylase, alpha subunit [EC: 4.1.1.3] (inferred from 93% identity to psa:PST_1377)

Predicted SEED Role

"Oxaloacetate decarboxylase alpha chain (EC 4.1.1.3)" in subsystem Na+ translocating decarboxylases and related biotin-dependent enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.3

Use Curated BLAST to search for 4.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNY0 at UniProt or InterPro

Protein Sequence (592 amino acids)

>Psest_2980 oxaloacetate decarboxylase alpha subunit (Pseudomonas stutzeri RCH2)
MTVAKQPLGITDVVLRDAHQSILATRVRLEDMLPIAAKLDQVGFWSVESWGGATFDACIR
YLGEDPWERIRELKKAMPNTRQQMLLRGQNLLGYRHYADDVVEKFVERAAVNGVDVFRVF
DAMNDPRNLETALRAVKQQGKHAQGTISYTTSPVHTLEMWVDLAKQIEDMGADSVAIKDM
AGILTPYMAFELVSRLKASLAIPIHMQCHATAGLSTAAILKAVEAGIDNVDTAISSLSMT
YGHSPTESVVAIFQGTDRDTRLNLELLEEIAAYFREVRKKYAKFEGTLRGVDSRILVAQV
PGGMLTNMEGQLKEQGALDKFDDVLAEIPRVREDLGFIPLVTPTSQIVGTQAVINVLTGE
RYKSITKETAGVLKGEYGAAPAPFNSALQARVLDGAEVITCRPADLLQPEMERLTVELKG
LAQEKNITLAADSIDDVLTYALFPQIGLKFLENRGNPAAFEPAPNGSDLPAREPGKPEVY
TVDVNGKSFVVQVSDGGDIEGIKPLGGAPANAASTAAVAPTSGEPQAAPLAGNIFKVLVQ
PGQLVQEGDLVIILEAMKMETEIRAFKAGTVASVNVKVGDAVSVGDSLLSIA