Protein Info for PGA1_c29100 in Phaeobacter inhibens DSM 17395

Annotation: 2,4-dienoyl-CoA reductase FadH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 675 PF00724: Oxidored_FMN" amino acids 7 to 330 (324 residues), 272 bits, see alignment E=4.5e-84 PF07992: Pyr_redox_2" amino acids 376 to 647 (272 residues), 91.8 bits, see alignment E=3.1e-29 PF01266: DAO" amino acids 377 to 411 (35 residues), 34.6 bits, see alignment (E = 9.2e-12) PF00890: FAD_binding_2" amino acids 377 to 416 (40 residues), 29.6 bits, see alignment 2.3e-10 PF00070: Pyr_redox" amino acids 377 to 411 (35 residues), 22.2 bits, see alignment (E = 8.9e-08) PF12831: FAD_oxidored" amino acids 377 to 415 (39 residues), 30.4 bits, see alignment 1.4e-10 PF13450: NAD_binding_8" amino acids 379 to 414 (36 residues), 36.4 bits, see alignment 2.8e-12

Best Hits

Swiss-Prot: 58% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 86% identity to sit:TM1040_2458)

MetaCyc: 58% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7F037 at UniProt or InterPro

Protein Sequence (675 amino acids)

>PGA1_c29100 2,4-dienoyl-CoA reductase FadH (Phaeobacter inhibens DSM 17395)
MTAYPKMLAPLDLGFTTLKNRVLMGSMHTGLEETKDWNRVAEFYADRARGGVALMVTGGI
GPNLEGSVLPGAAMMTTDQDVENHSIVTNRVHEAGGKIAMQILHAGRYSYGPKCVAPSPV
KSPISPFPPNELDEEGIEKQIADIVNAAVLAQQAGYDGVEIMGSEGYFLNQFLVTHTNKR
EDRWGGSYQNRMRLPIEVVRRTREAVGTDFIIIYRLSMIDLVPNGSTYDEVVQLAQEIEK
AGATILNTGIGWHEARIPTIATSVPRAAFAWVTKKLMGKVGIPVITSNRINTPEVAEQVL
SEGCADMVSMARPMLADADFVAKAADGKADHIAPCIACNQACLDHTFSGKLTSCLVNPRA
CHETELVLTPATTTKTVAIVGAGPAGLSTALAAAERGHKVTLFDRASEIGGQLNMAKQVP
GKEEFWGLVDWYRTMLAQSDVTLELNREVSAGDLKDFDEVVIATGVVPRDPQIPGQDRDN
VVDYIDVLRHKAEVGKRVAVIGAGGIGFDVSEFLLHEGESATENLPLWMKEWGVADPAEH
RAGLAPEGPQPEAPAREVTLLQRKAERHGKRLGKTTGWIHRATLKMKDVNFVGGVNYEKI
DDDGLHVSFGEARENPTVIAADTVVLCSGQLSERSLADALEAQGTSCHVIGGADLAAELD
AKRAINQGTRLAASL