Protein Info for Psest_2917 in Pseudomonas stutzeri RCH2
Annotation: 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K06182, ribosomal large subunit pseudouridine synthase F [EC: 5.4.99.12] (inferred from 85% identity to psa:PST_1452)Predicted SEED Role
"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)
MetaCyc Pathways
- pseudouridine degradation (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12
Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GPV8 at UniProt or InterPro
Protein Sequence (241 amino acids)
>Psest_2917 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases (Pseudomonas stutzeri RCH2) MTDPVRLAKRLAEQLGCSRREAELYIEGGWVTVDGELVEAPFLKVSPQQRIELRDGATAR EIAPVTLLLHKPAGLAIDVDADSVRRVLVASEHWSDDPSRIEPLQRHLLQQNILLPLDPE ASGLTVFSQQREVIRLLTSTRDKLEQEYVVEVSGEAEENGFALLAKGIGHRGKILPKAKV SWQSENRLRLVIKEPAPGEVRLLCEAIGLRVLACKRIRIGRLSMAKLPVGHWRIVGEHER F