Protein Info for PGA1_c02980 in Phaeobacter inhibens DSM 17395

Annotation: glutamate-cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 TIGR01436: glutamate--cysteine ligase" amino acids 10 to 456 (447 residues), 654.4 bits, see alignment E=3.3e-201 PF04107: GCS2" amino acids 62 to 358 (297 residues), 305.7 bits, see alignment E=1.7e-95

Best Hits

Swiss-Prot: 54% identical to GSH1_MEDTR: Glutamate--cysteine ligase, chloroplastic (GSH1) from Medicago truncatula

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 89% identity to sil:SPO3626)

MetaCyc: 54% identical to gamma-glutamylcysteine synthetase (Lotus japonicus)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DLK5 at UniProt or InterPro

Protein Sequence (456 amino acids)

>PGA1_c02980 glutamate-cysteine ligase (Phaeobacter inhibens DSM 17395)
MSIPQSGGGPIERHEQLAEYLASGCKPRDQWRIGTEHEKFGYCKDSLKPLPFEGTRSIVA
VLEGLRDRHGWSEVREGGHLIGLEKDGANVSLEPGGALELSGAPLETIHETCDEVNTHLR
EVKDIADEIGVGFIGLGAAPIWSHEEMPLMPKGRYKLMDAYMGKVGTMGRTMMRRTCTVQ
VNLDFGSEADMVQKMRVAIALQPVATALFANSPFLEGKPNNHKSWRSRVWRDLDADRTGM
VPFVFDEGFGFERWVEYALDVPMYFVYRDGQYIDALGMSFRDFLKGELPALPGQTPTLSD
WADHLTTAFPEARIKKYMEMRGADGGPWRRLCALPAFWVGMMYDQSSLDAAWDLAKGWDA
ETREALRVAASEQGLQAQVGNIKMHDLAREVVAISEAGLKSRARAGAGGLVPDETHFLNA
LKDSIETGKVPADELLDRYHGDWNGDLSRIYSEYSY