Protein Info for Psest_2891 in Pseudomonas stutzeri RCH2

Annotation: TonB-dependent siderophore receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 717 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF07715: Plug" amino acids 68 to 166 (99 residues), 76.7 bits, see alignment E=1.9e-25 TIGR01783: TonB-dependent siderophore receptor" amino acids 69 to 715 (647 residues), 364.3 bits, see alignment E=7e-113 PF00593: TonB_dep_Rec_b-barrel" amino acids 239 to 686 (448 residues), 244.8 bits, see alignment E=3.3e-76

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 77% identity to pmy:Pmen_4103)

Predicted SEED Role

"Ferrichrome-iron receptor" in subsystem Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPT3 at UniProt or InterPro

Protein Sequence (717 amino acids)

>Psest_2891 TonB-dependent siderophore receptor (Pseudomonas stutzeri RCH2)
MRRLLLPMLAVPVLTCPAMASANDEPPAEPLALEEMQVTASAERADGPVQGYRATRAASA
TRTDTAIHETPQSISVVPAQVVEDIGATRLEDALDYAGGVERGNNFGGQGLTEFLVRGFS
TQEFYRNGFAVNRGYPNMPDANTLERIEVLRGPASMLYGRGDPGGTFNIVSKQPQAERRT
VLGSQVNTDGLRRGTLDTTGALDESAAFTYRLNLVAEGSDSFRDHVESERYNIAPVLRWQ
LSDDTALILEGDYLHNRHPMDRGLPRYHNQAGNLPRDRFLGEESAGKLTNQNATTQLRLE
HQLDSQWMLRGGIQYLDGSLDGGAVENNGLPTTAVSADGRTVGRNYSERWLNWNDLAVQA
NLEGHFDAAGLAHTLLLGVEFDEYNYDSLIRRSGGSTTDFPIDLYDPVYGQPLPALTRTT
TYDDENLKSYAFFLQDQIALTERLKAQIGARLERFEQTYVDKRPGEKDWDQAHNAVSPRF
GLIYDLTEALAVYANTSRSFKPNPGADRSSQAFDPEKGVAHEIGIKYDLPERDLSVTAAL
FHITKENVLTDDPANTGAQSFKVAAGEARSRGFDISVAGNITPQWRVIGGYAYVDAEVTE
STSPSMPVGTRLANVPRHSFNLLDTYEFDEGPLAGLGVGMGVKYVGERKGSTSNSAFDMG
AYTTVDLLAYYPLTERVRLNLNLNNLFDEEYEERAWGNIWAYPGEPRTLQAGISVTL