Protein Info for HP15_281 in Marinobacter adhaerens HP15

Annotation: non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 65% identical to IXTPA_ECOL6: dITP/XTP pyrophosphatase (rdgB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01516, nucleoside-triphosphatase [EC: 3.6.1.15] (inferred from 80% identity to maq:Maqu_0529)

MetaCyc: 64% identical to dITP/XTP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Nucleotide diphosphatase. [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]

Predicted SEED Role

"Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15)" in subsystem Heat shock dnaK gene cluster extended (EC 3.6.1.15)

MetaCyc Pathways

superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
UTP and CTP dephosphorylation I (5/7 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
dZTP biosynthesis (3/5 steps found)
pyrimidine deoxyribonucleotides dephosphorylation (1/3 steps found)
tunicamycin biosynthesis (3/9 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.66 or 3.6.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PL03 at UniProt or InterPro

Protein Sequence (199 amino acids)

>HP15_281 non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family (Marinobacter adhaerens HP15)
MTNRLVIASNNRGKIAELTELLAPLGMTPIAQGDLGVGEAEEPAVTFVENAILKARHAAR
ETGLPALADDSGLAVDALEGRPGVRSARFAGDDATDQDNVDALLDAMAGVPDGQRGAQFH
CVLVYLRHAEDPTPIICHGRWPGSILRSPQGDGGFGYDPVFLAPEHGCSAAELSRAEKGS
ISHRGRALKILLDQLKAEA