Protein Info for PGA1_c28230 in Phaeobacter inhibens DSM 17395

Annotation: dimethylglycine dehydrogenase DmgdH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 859 PF00890: FAD_binding_2" amino acids 30 to 244 (215 residues), 30.1 bits, see alignment E=1.1e-10 PF01266: DAO" amino acids 30 to 404 (375 residues), 207.5 bits, see alignment E=1.6e-64 PF16350: FAO_M" amino acids 407 to 461 (55 residues), 53.9 bits, see alignment 5.8e-18 PF01571: GCV_T" amino acids 466 to 745 (280 residues), 221 bits, see alignment E=5.9e-69 PF08669: GCV_T_C" amino acids 769 to 849 (81 residues), 54.4 bits, see alignment E=3.4e-18

Best Hits

KEGG orthology group: K00315, dimethylglycine dehydrogenase [EC: 1.5.99.2] (inferred from 97% identity to sit:TM1040_0486)

Predicted SEED Role

"Sarcosine dehydrogenase (EC 1.5.99.1)" (EC 1.5.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.99.1, 1.5.99.2

Use Curated BLAST to search for 1.5.99.1 or 1.5.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E3Y1 at UniProt or InterPro

Protein Sequence (859 amino acids)

>PGA1_c28230 dimethylglycine dehydrogenase DmgdH (Phaeobacter inhibens DSM 17395)
MAQNLQAGHNSLQTSHHFPESGPMKTQVKALVVGGGAVGTSIAYHLAKAGWDDVMLIERD
ELTSGSTWHAAGLLPLFNMSYATTHIHKYSVDFYKTLEEETGLNAGFAVVGNLRMAQTQD
RMDEYMLYASTAETCDVDYEWLTPDQIKERWPLIETGDLKGAIYHTEDGYINPADVTQAM
AKGARQRGVEIHRKLQADDFHWNGTHWEVTCTKMIEKGGNLIESDEKVVITAEHVVTASG
NHAQRTAKMLGIKMPAIPVEHTFIVMDKDPELVKWREAGNPEHPVIRDADNESYAREERG
GWILGIYEHGAPARFEHGVPDSFRADLFPLDLDRIADQYMAMAERVPSCAESGLKDDFNG
PICYTPDGNPLVGPAPGLRNMWLAEGFSFGITAAGGTGYYLAQMMVDGEAEIDMASLDPK
RYSSNWMTTEFAARKNEECYEHVYILHHPDEERPAARPLRTAPAFDRQKARGAQFGWVNG
WERPNYFGPVDAPDNFDHDARSFRRGGWWQHAVDEAKAIREGVGLVDATAFTKHVVKGPG
ATQFLDWFTCNKLPKVGRINLTYALTAFGTTRTEYTIVRNGENNYYLVSAGAWSEYDADF
LRKAAEDKMEEFGYIEIQDVTTQWGVFAIAGPKSRDVLKEVIVDADPETALSNKRFPWLS
ARQIELGMCPVNAIRVAYTGELGWELHHPIEMQNYLFDLLEKAGEKHGMKLVGARAQNWL
RQEKSYRAFGNELGRDATPLEADLPRFVDLEKDFHGKDKLVETGVRVKCCTLLIDGPEDA
DPWGHEALYAEDGVTRVGRLTSGGYSVAFEKSIGMGYVKPDVAVEGTKLKVKMQDKLWDA
VVTCDSPYDPKNETIRQNG