Protein Info for Psest_2731 in Pseudomonas stutzeri RCH2

Annotation: ribonucleoside-diphosphate reductase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 27 to 122 (96 residues), 65 bits, E=1.1e-21
  • amino acids 143 to 227 (85 residues), 29 bits, E=1.9e-10
• PF00317: Ribonuc_red_lgN
  • amino acids 288 to 360 (73 residues), 85.1 bits, 4.4e-28
• TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit
  • amino acids 291 to 901 (611 residues), 706.2 bits, E=1.9e-216
• PF02867: Ribonuc_red_lgC
  • amino acids 365 to 900 (536 residues), 634.9 bits, E=1.6e-194

Best Hits

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 74% identity to abo:ABO_0711)

Predicted SEED Role

"Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• superpathway of purine nucleotide salvage (11/14 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

See L0GKG0 at UniProt or InterPro

Protein Sequence (960 amino acids)

>Psest_2731 ribonucleoside-diphosphate reductase, alpha subunit (Pseudomonas stutzeri RCH2)
MQNPSTRENPLSADAPDLAATAPGQLRVIKRNGTVVPYTDDKITVAITKAFLAVEGGNAA
ASSRIHDTVARLTEQVSATFRRRMPSGGTIHIEEIQDQVELALMRAGEQKVARDYVIYRE
AQAVKRKSASDSSDIAQPHPSIRVTRADGSLQPLDMGRLQTIITEACEGLAEVDGALIER
ETLKNLYDGVAEKDVNTALVMTARTLVEREPNYSQVTARLLMDTLRAEALGFLGVAEAAT
HHEMADLYAKALPAYIEKGVEFELLDSKLKGYDLAKLGAALNHERDQQFTYLGLQTLYDR
YFIHKDNVRFELPQIFFMRVAMGLAIEEEQKEARAIEFYNLLSSFDYMASTPTLFNAGTL
RPQLSSCYLTTVPDDLGGIYDAIRDNALLSKFAGGLGNDWTPVRALGAYIKGTNGKSQGV
VPFLKVVNDTAVAVNQGGKRKGAVCAYLETWHLDIEEFLELRKNTGDDRRRTHDMNTANW
IPDLFMKRVFDDGPWTLFSPSDVPDLHDLTGRAFEERYEYYEELTKYGKIKLFKTLPAKD
LWRKMLSMLFETGHPWLTFKDPCNLRSPQQHVGVVHSSNLCTEITLNTNKDEIAVCNLGS
INLVNHIKDGKLDAAKLERTVRTAVRMLDNVIDINYYSVPQAQNANFKHRPVGLGIMGFQ
DALYLQHIAYGSDAAIDFADKSMEAISYFAIQASCDLADERGAYSSFQGSLWSQGILPLD
SQQILIEARGQKYIDVDLTESLDWAPIRERVKKGIRNSNIMAIAPTATISNIVGVSQSIE
PTYQNLYVKSNLSGEFTVINPYLVHDLKARGLWDPVMVNDLKYYDGSVQQIERIPQDLKD
MYATAFEVETKWIVDAASRRQKWIDQAQSLNLYIAGASGKKLDVTYRMAWYRGLKTTYYL
RALAATSTEKSTINTGKLNAVSSGGSEAANSASAAPAAEPKPAPVPLACSIDNPDCEACQ