Protein Info for PGA1_c26800 in Phaeobacter inhibens DSM 17395
Annotation: phenylacetate-CoA ligase PaaK
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to PAAK_PSEPU: Phenylacetate-coenzyme A ligase (paaK) from Pseudomonas putida
KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 94% identity to sil:SPO0742)MetaCyc: 72% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]
Predicted SEED Role
"Phenylacetate-coenzyme A ligase (EC 6.2.1.30) PaaF" (EC 6.2.1.30)
MetaCyc Pathways
- phenylacetate degradation I (aerobic) (6/9 steps found)
- penicillin G and penicillin V biosynthesis (1/4 steps found)
- phenylacetate degradation II (anaerobic) (1/4 steps found)
- superpathway of phenylethylamine degradation (6/11 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (4/9 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (4/27 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.30
Use Curated BLAST to search for 6.2.1.30
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7E3M8 at UniProt or InterPro
Protein Sequence (436 amino acids)
>PGA1_c26800 phenylacetate-CoA ligase PaaK (Phaeobacter inhibens DSM 17395) MEDLSPKKGELDPIEIASIDEIRSLQLDRLKWSLRHAYDNVAMYKQRFDEAGVHPDDLQQ LSDLAKFPFTYKNDLRDNYPFGLFAVPREQIMRLHASSGTTGKPTVVGYTANDISNWADL VARSLRASGLRKGDMVHNAYGYGLFTGGLGAHYGIERLGATVVPMSGGQTEKQVGLITDF KPDGIMVTPSYMLNILEQYHKVGMDPRECSLKVGVFGAEPWTDAMRREVEQAFDMHAVDI YGLSEIMGPGVANECVETKDGPVIWEDHFLPEIIDPQTGEVLPDGEMGELVFTTLTKEGL PMVRYRTRDLTRLLPGTARSMRRMEKITGRSDDMIILRGVNVFPSQVEEQLLATGGLAPY YQIELYKSGRMDAMRVFVEANPDATDELSKTAAARMLTKRIKDMVGVSTEIIVGEPGSVE RSQGKAKRVVDNRSKE