Protein Info for PGA1_c26570 in Phaeobacter inhibens DSM 17395

Annotation: putative glyoxylate/hydroxypyruvate reductase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02826: 2-Hacid_dh_C
  • amino acids 112 to 273 (162 residues), 140.7 bits, E=5.1e-45
• PF03446: NAD_binding_2
  • amino acids 135 to 222 (88 residues), 31.3 bits, E=3e-11

Best Hits

KEGG orthology group: K12972, gyoxylate/hydroxypyruvate reductase A [EC: 1.1.1.79 1.1.1.81] (inferred from 75% identity to sit:TM1040_0466)

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-serine biosynthesis II (3/4 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.79, 1.1.1.81, 1.1.1.95

Use Curated BLAST to search for 1.1.1.79 or 1.1.1.81 or 1.1.1.95

Sequence Analysis Tools

See I7F1Y5 at UniProt or InterPro

Protein Sequence (308 amino acids)

>PGA1_c26570 putative glyoxylate/hydroxypyruvate reductase A (Phaeobacter inhibens DSM 17395)
MINVQFAALPERWATYETPLRAAFAEAGLKVDLRLDHTPDEVDYVVYAPNSDLQDFTPYT
RCKAVLSLWAGVEKIVGNRTLTMPLCRMVDPGLTAGMVEWVTGHVLRYHLNIDRTIHTQD
RWEPVVPPLAEERPVTVLGLGALGQACASMLATLGFPVTGWSRSKKSIDGITCRHGDDGL
RDALATAQIVILLLPDTHATENTLNSDTLALLPKGARIINPGRGPLIDDDALLAALDSGQ
IGHATLDVFRIEPLPMDHPYWAHPRVTVTPHIAAETRPLTAARVIADNIRRGEAEAPFLH
QVDRTLGY