Protein Info for Psest_2661 in Pseudomonas stutzeri RCH2

Annotation: Isopropylmalate/homocitrate/citramalate synthases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00682: HMGL-like
  • amino acids 5 to 276 (272 residues), 158.6 bits, E=1.1e-50

Best Hits

Swiss-Prot: 45% identical to HMGCL_BACSU: Hydroxymethylglutaryl-CoA lyase YngG (yngG) from Bacillus subtilis (strain 168)

KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 94% identity to psa:PST_1716)

MetaCyc: 38% identical to Hydroxymethylglutaryl-CoA lyase, mitochondrial (Homo sapiens)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• mevalonate degradation (1/2 steps found)
• ketogenesis (3/5 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.4

Use Curated BLAST to search for 4.1.3.4

Sequence Analysis Tools

See L0GME3 at UniProt or InterPro

Protein Sequence (311 amino acids)

>Psest_2661 Isopropylmalate/homocitrate/citramalate synthases (Pseudomonas stutzeri RCH2)
MNKRLYIQEVATRDGFQIEADFVPTEAKIALIDRLSQTGLAKIEVTSFTSPKAIPNLRDA
EDVMRGIRRVEGVEYTVLVPNVKGCERALACEVDEINLVMSASDTHGLANLRMTPEQSLV
QFREIIEVTRGSGVFINASLSTTFGCPFEGDVPERRIYELVQRLLEIGVQGITLCDTTGM
ADPAQVERICHEVLNSWPQAVFTAHFHNTRGMGLANALAALNAGIDRFDASLGGLGGCPY
APGASGNICTEDLVHMFQRMGLNTGVDLDRLLQCAADLPQLVGHDVPGAVLKAGKADRRY
PKPKWMQEAGV