Protein Info for Psest_2649 in Pseudomonas stutzeri RCH2

Annotation: Predicted permease, DMT superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 279 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 36 to 54 (19 residues), see Phobius details amino acids 65 to 84 (20 residues), see Phobius details amino acids 90 to 109 (20 residues), see Phobius details amino acids 116 to 135 (20 residues), see Phobius details amino acids 141 to 160 (20 residues), see Phobius details amino acids 170 to 191 (22 residues), see Phobius details amino acids 201 to 221 (21 residues), see Phobius details amino acids 229 to 250 (22 residues), see Phobius details amino acids 256 to 275 (20 residues), see Phobius details PF00892: EamA" amino acids 6 to 133 (128 residues), 39.7 bits, see alignment E=2.9e-14 amino acids 144 to 273 (130 residues), 49.8 bits, see alignment E=2.2e-17

Best Hits

Swiss-Prot: 63% identical to Y1977_PSEAE: Uncharacterized protein PA1977 (PA1977) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 82% identity to psa:PST_1731)

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GP57 at UniProt or InterPro

Protein Sequence (279 amino acids)

>Psest_2649 Predicted permease, DMT superfamily (Pseudomonas stutzeri RCH2)
MRPRTLLLTALAMLAFAGNSILCRIALRDTTIDPASFTGLRILAGALTLWLLLRLSRDRR
STGGDWLSALALFVYAASFSYAYINLDAGAGALLLFGAVQLSMLAWGVLRGERFSAGQIA
GLLLAMSGLLILLLPGSRAPALDGALLMLLSGVAWGIYSLRGRGASNPLAATAGNFIKAV
PFAAVLSLVMLGQQQWDAPGVVYALLSGALASGIGYAIWYAALPGLAAIQAASVQLSVPL
LTAVAGAVLLGEALTATLIISGAAILGGIAMVLSIRHRS