Protein Info for Psest_2632 in Pseudomonas stutzeri RCH2

Annotation: ribosomal protein S12 methylthiotransferase RimO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 6 to 437 (432 residues), 344.8 bits, see alignment E=7e-107 TIGR01125: ribosomal protein S12 methylthiotransferase RimO" amino acids 6 to 436 (431 residues), 589 bits, see alignment E=6.5e-181 PF00919: UPF0004" amino acids 7 to 84 (78 residues), 72.2 bits, see alignment E=6.2e-24 PF04055: Radical_SAM" amino acids 143 to 321 (179 residues), 84.1 bits, see alignment E=2.7e-27 PF01938: TRAM" amino acids 376 to 440 (65 residues), 21.7 bits, see alignment E=2.9e-08 PF18693: TRAM_2" amino acids 378 to 440 (63 residues), 84.7 bits, see alignment E=8e-28

Best Hits

Swiss-Prot: 98% identical to RIMO_PSEU5: Ribosomal protein S12 methylthiotransferase RimO (rimO) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K14441, ribosomal protein S12 methylthiotransferase [EC: 2.-.-.-] (inferred from 98% identity to psa:PST_1747)

MetaCyc: 72% identical to ribosomal protein S12 methylthiotransferase RimO (Escherichia coli K-12 substr. MG1655)
RXN0-6366 [EC: 2.8.4.4]

Predicted SEED Role

"Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.8.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GK81 at UniProt or InterPro

Protein Sequence (440 amino acids)

>Psest_2632 ribosomal protein S12 methylthiotransferase RimO (Pseudomonas stutzeri RCH2)
MSKTPTVGFVSLGCPKATVDSERILTQLRMEGYQIVPSYEDADVVVVNTCGFIDSAKAES
LDAIGEAIAENGKVIVTGCMGVDENNIRGVHPSVLAVTGPQQYEQVVNAVHEVVPPNIEH
DPFVDLVPPQGIKLTPRHYAYLKISEGCNHTCSFCIIPSMRGKLVSRPVGDVLSEAERLV
KAGVKEVLVISQDTSAYGVDLKYKLDFWNGQPVKTRMLELCEELGKMGVWVRLHYVYPYP
NVDDVIPLMAAGKILPYLDIPFQHASPKVLKAMKRPAFEDKTLARIKKWREICPELTIRS
TFIVGFPGETEEDFQYLLDWLTEAQLDRVGCFQYSPVDGAPAEVMGLEAVPDEIKQERWD
RFMAHQQAISAARLQLKVGQEMDVLIDEVDEDGAIGRSWADAPEIDGMVYVDSAQPLQPG
DKVRVRVTNADEYDLWAEVI