Protein Info for Psest_2625 in Pseudomonas stutzeri RCH2

Annotation: Flp pilus assembly protein, secretin CpaC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF13629: T2SS-T3SS_pil_N" amino acids 45 to 113 (69 residues), 71.7 bits, see alignment E=3.2e-24 PF00263: Secretin" amino acids 283 to 450 (168 residues), 145.6 bits, see alignment E=1.2e-46

Best Hits

Predicted SEED Role

"Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMB2 at UniProt or InterPro

Protein Sequence (504 amino acids)

>Psest_2625 Flp pilus assembly protein, secretin CpaC (Pseudomonas stutzeri RCH2)
MNSMSKVQLLAVLLGGWAVLGAPATFAQQPVSATAVRAMPAVNNSDVQVPIYKSRVLSTR
ASVNRISVGNPAIADILITSPTQLYLLGRSLGSTNVLLWDNRNQLIDSLDVEVVHDLGGL
KAKLHQVLPNERIEVFSAQGALALRGQVSSAAAMDSAVKLAQSYAAQASAAESGGAAMQN
KPLEVINLLGIGGSQQVMLEVKVAEMQRSLFKNLDVRFNALDFGSSSRWSGGGFNNGLNA
DGGVLGLNPDGQVDPSKLIGSGKAIFGQFLSENFLFNVVLDAAKDNGTAKVLAEPTLTTL
TGQQAEFISGGEFPVPVSDDDGITIEYKEFGVGVKFLPVVLDSGRINLNLNVAVSELSNA
NALMLTTGLGGTNSVGQLVPSLTKRSAQSTVELGNGQTIAIAGLISENSRDYVSRFPGLG
DIPVLGQLFRSQQYINGETELVILVTPHLVTPLDAKAVRLPTENFVEPSDLEFYLLGKTK
GSQPGRAVPVSLGVSEGRFGHDLK