Protein Info for HP15_2498 in Marinobacter adhaerens HP15

Annotation: small GTP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 10 to 360 (351 residues), 511.2 bits, E=6.1e-158
• PF13167: GTP-bdg_N
  • amino acids 28 to 112 (85 residues), 106.8 bits, E=1.4e-34
• PF16360: GTP-bdg_M
  • amino acids 114 to 192 (79 residues), 105.3 bits, E=4.1e-34
• PF01926: MMR_HSR1
  • amino acids 200 to 318 (119 residues), 74.9 bits, E=1.2e-24
• PF19275: HflX_C
  • amino acids 341 to 425 (85 residues), 31.9 bits, E=2.2e-11

Best Hits

Swiss-Prot: 64% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 90% identity to maq:Maqu_2768)

MetaCyc: 64% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See E4PHX4 at UniProt or InterPro

Protein Sequence (432 amino acids)

>HP15_2498 small GTP-binding protein (Marinobacter adhaerens HP15)
MFERPDVGERAVLVHIDFTAHDGTEDPGEFRELVTSAGVEPVSTVTGSRKQPSPRFFVGE
GKLEEIRDAVAANEADVVLFNHALSPRQERNIEHELKCRVLDRTGVILDIFAQRARTHEG
KLQVELAQLEHMSTRLVRGWTHLERQKGGIGLRGPGETQLETDRRLLRERIKSIHKRLEK
VRRQRNQGRRARQRADIPTVSLVGYTNAGKSTLFNRITTSSVYAADQLFATLDPTLRRLE
LPDVGPVVMADTVGFIRHLPHKLVEAFRATLEETTQATLLLHIIDSHDPRREENIEQVEE
VLAEIGADDIPMLQVFNKIDLLENFTPRVERNEEGIPVRAWVSAVTGEGLDGLFDAIVER
VAEDVVHHFVLLGPADGKLRALLHEAGSVISEEYRDTGDTVVEVRLQNRDWHQLLSRAGV
NEKSVRLDVGHA