Protein Info for PGA1_c25740 in Phaeobacter inhibens DSM 17395

Annotation: formate dehydrogenase H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 924 PF13510: Fer2_4" amino acids 3 to 81 (79 residues), 72.5 bits, see alignment E=1e-23 PF13183: Fer4_8" amino acids 148 to 207 (60 residues), 28.4 bits, see alignment 8.1e-10 PF12838: Fer4_7" amino acids 151 to 208 (58 residues), 33.2 bits, see alignment 2.4e-11 PF00037: Fer4" amino acids 192 to 209 (18 residues), 25 bits, see alignment (E = 5e-09) PF04879: Molybdop_Fe4S4" amino acids 229 to 280 (52 residues), 64.4 bits, see alignment 3e-21 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 233 to 914 (682 residues), 736.5 bits, see alignment E=1.2e-225 PF00384: Molybdopterin" amino acids 283 to 720 (438 residues), 248.4 bits, see alignment E=4.5e-77 PF01568: Molydop_binding" amino acids 805 to 908 (104 residues), 91.7 bits, see alignment E=1.2e-29

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 75% identity to dsh:Dshi_1282)

Predicted SEED Role

"tungsten-containing formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E3A8 at UniProt or InterPro

Protein Sequence (924 amino acids)

>PGA1_c25740 formate dehydrogenase H (Phaeobacter inhibens DSM 17395)
MSDKITFTLDGEQVTADAGLTIWEVANGRGLKIPHLCHKPQPGYRPDGNCRACMVEIEGE
RTLAASCIREPAEGMVVTTNNARAENARKMVMELLVADQPKKEEARDKSSHLWDMAELNG
VSESRFPKLEKDRIPLLDDSHVAMKVNLDACISCNLCVRACREVQVNDVIGMAGRGHDAY
PVFDIADPMGASSCVACGECVQACPTGALMPATVMDDNQVGDSKDYDSETESVCPFCGVG
CKVSLKVKDGKVKHVEGINGPANEGRLCVKGRFGFDYIHHPHRLTKPLIRRDDAPAKGLN
VDPGNLSTHFREATWEEAMDLAGKGLMKLRDEDPKSVAGFGSAKCTNEEAYLFQKFIRQG
FKHNNVDHCTRLCHASSVAALIENVGSGAVTATFNEIENADVAIVIGSNPIENHPVAATY
FKQFTKRGGKLIVMDPRGVGLRRFATEMVQFRPGADVSMLNAIMNVIVEEELYDSQYIHR
WTENWEAEKEHLKQFTPEKMSEICGIEPDQLRRVARIFAGGNAGLIFWGMGISQHIHGTD
NSRCLISLALMTGNVGKPGAGLHPLRGQNNVQGASDAGLIPMFLPDYQTVTSDDVRKSFT
DVWGGGDFSNEKGLTVTEIVDQAYAGNIKGMYIQGENPAMSDPDADHAREAFAKLELMIV
QDIFLTETANYADIILPASALYEKNGTVSNTNRQVQRVRPAVAPPGEAREDWKVTVELAR
RIGLPWDYKDVSEVFNEMKLNMKSLNNITWERLETETITYPSLHETDPGQAIVFGDGFPR
PEGRARFTPASVIPPDEAPDAEYPMIMTTGRQLEHWHTGSMTRRSLVLDAVEPEANCSLN
PRTLKLMGVEPGEMVRLSTRRGSIEIMARADRAIAEDMVFVPFAYVEAAANILTNPALDP
YGKIPEFKFSAVKVEKIEGQIAAE