Protein Info for HP15_2478 in Marinobacter adhaerens HP15
Updated annotation (from data): Acyl-CoA dehydrogenase (EC 1.3.8.7)
Rationale: Specifically important for: Tween 20. tween 20 hydrolyzes to a mix of C12, C14, and C16 fatty acids; this is probably part of beta oxidation (SEED_correct)
Original annotation: acyl-CoA dehydrogenase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 90% identity to maq:Maqu_2749)Predicted SEED Role
"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)
MetaCyc Pathways
- oleate β-oxidation (30/35 steps found)
- fatty acid salvage (6/6 steps found)
- adipate degradation (5/5 steps found)
- 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Brassinosteroid biosynthesis
- Caprolactam degradation
- Fatty acid metabolism
- Geraniol degradation
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-
Use Curated BLAST to search for 1.3.8.7 or 1.3.99.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E4PHV4 at UniProt or InterPro
Protein Sequence (586 amino acids)
>HP15_2478 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Marinobacter adhaerens HP15) MRFVLNEVFDAPALWASLPKVAEHVDPETADAILEEAGKISSGVLAPLNREGDEQGCKWN DGEVTSPEGFKEAYQTIVEGGWNGLGGNPDFGGMGMPKTLVAQFEEMMQGANMAFGLAPM LTAGACLALDAHGSDELKEKYLPNMYSGVWSGAMDLTEPHAGTDLGIIRTKAEPNDDGSF NVTGTKIFITWGEHDMAENIIHLVLAKLPDAPKGPKGISMFLVPKFLVNDDGSLGERNSF SCGSLEKKMGIKGSATCVMNFDGAKGWLVGEENKGLAAMFTMMNYERLGVGIQGIGAAEA SLQSAREYALDRIQSRAPTGAQQPEKAADPILVHPDVRRMLLTMKSYVEGGRTFSTYVAQ WLDIAKFAEDDERRKHAEGMVALLTPVAKAFLTDRGLDTCIMGQQVFGGHGFIREWGQEQ LVRDCRITQIYEGTNGIQALDLMGRKVVGTQGKLYELFAQDVANFLEENSGNDHLRPFLE PLAAAVERLDDVTEHVIKQAGDNPNAIGAASVDYLDLFGLTALGYMWAKIVKAAAPQADS DTSGFYSGKLKTARFYFDRLLPKTVSLAEGIRSGSDSMMALTAEEF