Protein Info for Psest_2582 in Pseudomonas stutzeri RCH2

Annotation: folylpolyglutamate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 25 to 420 (396 residues), 317 bits, E=1.2e-98
• PF08245: Mur_ligase_M
  • amino acids 47 to 186 (140 residues), 45.3 bits, E=5.2e-16

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 80% identity to psa:PST_1781)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)" in subsystem Folate Biosynthesis (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of chorismate metabolism (44/59 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See L0GNZ6 at UniProt or InterPro

Protein Sequence (434 amino acids)

>Psest_2582 folylpolyglutamate synthase/dihydrofolate synthase (Pseudomonas stutzeri RCH2)
MTERSLADWLAYLEQLHPTAIDMGLDRVRLVSARLGLTRSAPLAVTVTGTNGKGSTCAFI
ASLLNSQGMRVGTYSSPHLLRYNERVQLDGCEASDQELCEAFAAVEAARGEVSLTYFEMG
TLAAFWLFERAELDAAVLEVGLGGRLDAVNIVDADLAVITNIGLDHADWLGDTRESVAYE
KAGIMRAGKPVLCGDLDVPAPLLQQAESLKAPLLLRGRDFDLAFAPRAWHWRGVGPAGEP
LELHGLPLLSLPMENAAIALQAYASLGLPWDVKVLSAALEQTRVTGRLDRRQITWRGRKV
SLLLDVGHNPHAASYLAQRIEQRPIAGRRLAVFGLLADKDLDGVLDAVQASITDWAVAPL
PTSRTQSATQLSAALLERGALVGEYASIEQALDAQCAKAEPDDEVLVFGSFYCVAAALGW
LERQAEVGGDGDAR