Protein Info for Psest_2579 in Pseudomonas stutzeri RCH2

Annotation: amidophosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01134: amidophosphoribosyltransferase
  • amino acids 2 to 464 (463 residues), 536 bits, E=5e-165
• PF13522: GATase_6
  • amino acids 65 to 198 (134 residues), 49.8 bits, E=7.2e-17
• PF13537: GATase_7
  • amino acids 80 to 212 (133 residues), 37.7 bits, E=3.8e-13
• PF00156: Pribosyltran
  • amino acids 274 to 393 (120 residues), 31.9 bits, E=1.6e-11

Best Hits

Swiss-Prot: 90% identical to PUR1_PSEAE: Amidophosphoribosyltransferase (purF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 99% identity to psa:PST_1784)

MetaCyc: 64% identical to amidophosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Amidophosphoribosyltransferase. [EC: 2.4.2.14]

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

See L0GMT0 at UniProt or InterPro

Protein Sequence (501 amino acids)

>Psest_2579 amidophosphoribosyltransferase (Pseudomonas stutzeri RCH2)
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFQQRHM
QRLVGNMGIGHIRYPTAGSSSSAEAQPFYVNSPYGITLAHNGNLTNVDQLTKEIYESDLR
HVNTSSDSEVLLNVFAHELAVRGKLQPTEEDVFAAVSKVHERCRGGYAVVAMVTGYGIVG
FRDPNAIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITTDGKLFTR
QCADAPKLAPCIFEYVYLARSDSLMDGVSVYKARLRMGEKLADKILRERPEHDIDVVIPI
PDTSRTAALELANRLGVKFREGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIDLEFRGKN
VMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRYPNVYGIDMPSAHELIAHNRS
TEEVAELIGADWLVYQDLEDLIDAVGGGKVKIDNFDCAVFDGKYITGDIDEAYLHKIEQA
RNDLSKVKTNAVSAIIDLYNN