Protein Info for HP15_2473 in Marinobacter adhaerens HP15

Annotation: leucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 861 TIGR00396: leucine--tRNA ligase" amino acids 5 to 860 (856 residues), 1221 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 16 to 225 (210 residues), 108.3 bits, see alignment E=8e-35 amino acids 417 to 572 (156 residues), 34.1 bits, see alignment E=2.7e-12 amino acids 619 to 659 (41 residues), 29.3 bits, see alignment 7.9e-11 PF09334: tRNA-synt_1g" amino acids 39 to 171 (133 residues), 71.7 bits, see alignment E=1.1e-23 PF13603: tRNA-synt_1_2" amino acids 221 to 404 (184 residues), 261.6 bits, see alignment E=6.5e-82 PF08264: Anticodon_1" amino acids 700 to 822 (123 residues), 70.1 bits, see alignment E=3.9e-23

Best Hits

Swiss-Prot: 68% identical to SYL_AERS4: Leucine--tRNA ligase (leuS) from Aeromonas salmonicida (strain A449)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 68% identity to amc:MADE_01672)

MetaCyc: 65% identical to leucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Leucine--tRNA ligase. [EC: 6.1.1.4]; 6.1.1.4 [EC: 6.1.1.4]; 3.1.1.- [EC: 6.1.1.4]

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PHU9 at UniProt or InterPro

Protein Sequence (861 amino acids)

>HP15_2473 leucyl-tRNA synthetase (Marinobacter adhaerens HP15)
MDEQYNPRDVEQNARDFWEENKTFMVKEEPGKPKYYCLSMFPYPSGKLHMGHVRNYTIGD
VISRYQRMQGKNVMQPMGWDAFGLPAENAAIANKTAPAKWTYANIEYMKNQLKQLGFGYD
WDRELATCKPDYYRWEQWFFARLYEKGLVYKKMSTVNWDPVDQTVLANEQVVDGRGWRSG
ALVEQKKIPQWFIRITDYAEELLNDLDQLEDWPEQVKTMQRNWIGKSEGTELTFPLKDRD
SGLTVYTTRPDTLMGVSYMAVAAEHPLAKEAAERHRDVAEFVEECRNSKTAEAELATMEK
RGIDTGFKAIHPLTQEEIPIWVANFVLTDYGTGALMAVPGHDERDHEFARKYKLPIKQVI
AARDGREIDVQEEAFSEKGTLVSSGKYSGLTSEEAFDEIASYLEEQNIGKRTVNYRLRDW
GVSRQRYWGAPIPMMTLEDGTEMPVPDDKLPVRLPEDVEMDGVQSPIKADPEWCKTEFNG
QPATLETDTFDTFMESSWYYARFCSPNYDQGMLDPSAANYWLPVDQYIGGIEHAILHLLY
ARFFHKLLRDVGLVTSSEPFNRLLCQGMVLAETYYRDDGKGGKVWISPADVTVERDEKGQ
PVRAVHREDGEPVVSGGVTKMSKSKNNGIDPQAIIDEHGADTVRLFMMFAAPPEQSLEWS
DSGVEGAHRFLKRVWRLVNEHAVAGPAPALDAASLNDDQKNLRRKTHETIAKVSDDISRR
LTFNTAIAAVMELLNEVGKLQGDEPQTRAVRQEALDTAVLVLSPIVPHICHTLWQALGHQ
EPVVDAPWPKADEAAMVRSQIQVVLQVNGKVRAKEDVPADISKADLEKLALENENVMRFT
EGATVRKVIVVPGKLVNVVAN