Protein Info for Psest_2560 in Pseudomonas stutzeri RCH2

Annotation: Zinc metalloprotease (elastase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 PF16485: PLN_propep" amino acids 4 to 45 (42 residues), 66.4 bits, see alignment 2.2e-22 PF01447: Peptidase_M4" amino acids 89 to 172 (84 residues), 100.6 bits, see alignment E=1.7e-32 PF02868: Peptidase_M4_C" amino acids 175 to 344 (170 residues), 180.1 bits, see alignment E=5e-57

Best Hits

Swiss-Prot: 65% identical to PRT1_PECCC: Extracellular metalloprotease (prt1) from Pectobacterium carotovorum subsp. carotovorum

KEGG orthology group: None (inferred from 88% identity to psa:PST_1806)

Predicted SEED Role

"peptidase M4, thermolysin"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMR8 at UniProt or InterPro

Protein Sequence (347 amino acids)

>Psest_2560 Zinc metalloprotease (elastase) (Pseudomonas stutzeri RCH2)
MQIRSFIPPYILDRIIDHGSPYQRGCARQTLDHVHSLLPNPGPPRPTAIAELGELRTPGH
PQRRIHDAEQRMQLPGIPRRREGLPASGDAAVDEAYDALGATYTFFWQVFQRDSIDDLGM
PLIGTVHYGQGYENAFWNGEQMVFGDGDGELFQRFTRSLDVVAHELAHGVIESEAGLVYF
NQPGALNESISDVFGVLTKQHALGQTAAEADWLVGAELLTDKVSGVALRSMANPGTAYDD
PLLGRDPQPAHMRDFIETREDNGGVHLNSGIPNRAFYLVATALGGYAWEQAGRIWYATLC
DPQLPNDADFVTFSSATLAHAAKLFGRQSSQVQALHEAWTSVGVVPR