Protein Info for HP15_2409 in Marinobacter adhaerens HP15

Annotation: ATPase, type IV, pilus assembly, PilB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 571 TIGR02538: type IV-A pilus assembly ATPase PilB" amino acids 13 to 571 (559 residues), 843.5 bits, see alignment E=4.1e-258 PF05157: MshEN" amino acids 46 to 151 (106 residues), 58.8 bits, see alignment E=5.5e-20 PF00437: T2SSE" amino acids 194 to 461 (268 residues), 362.7 bits, see alignment E=1e-112

Best Hits

Swiss-Prot: 64% identical to PILB_PSEAE: Type 4 fimbrial assembly protein PilB (pilB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02652, type IV pilus assembly protein PilB (inferred from 92% identity to maq:Maqu_2681)

Predicted SEED Role

"Type IV fimbrial assembly, ATPase PilB" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PH70 at UniProt or InterPro

Protein Sequence (571 amino acids)

>HP15_2409 ATPase, type IV, pilus assembly, PilB (Marinobacter adhaerens HP15)
MATDSSKVTLTGLARRFVEDGLLDEATAKDAFIQASQNRIPLITYLVQNNLADSSGLAFS
AAMEFGVSVLDLSAFATEMIPEKVVDEKLIRKHNALPIYKRGNRLFIAVSDPTNIQALDE
FKFNTGLSTDAVLVDDAKLRDSIEKYLESQDTSMGDLEDADLEGVETEGGEQEDDNAVSA
SDVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKL
GIDVLGYEEDQKQIYLEALEQPQGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDP
AEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGEIRDLETANIAIKAAQTGHLV
LSTLHTNSAAETLTRMMNMGVPSFNIATSVSVIIAQRLGRRLCNSCKQAADIPKDVLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQ
AEGFRNLRQAALLKVIQGVTSLEEANRVTKD