Protein Info for Psest_2484 in Pseudomonas stutzeri RCH2

Annotation: Molecular chaperone, HSP90 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 PF02518: HATPase_c" amino acids 31 to 185 (155 residues), 29.7 bits, see alignment E=1.1e-10 PF13589: HATPase_c_3" amino acids 33 to 157 (125 residues), 37.4 bits, see alignment E=3.8e-13 PF00183: HSP90" amino acids 222 to 629 (408 residues), 444.6 bits, see alignment E=9.3e-137

Best Hits

Swiss-Prot: 93% identical to HTPG_PSEMY: Chaperone protein HtpG (htpG) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 96% identity to psa:PST_1885)

MetaCyc: 61% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GM07 at UniProt or InterPro

Protein Sequence (634 amino acids)

>Psest_2484 Molecular chaperone, HSP90 family (Pseudomonas stutzeri RCH2)
MSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAADKLRFEALAKPELL
EGGADLKIRLSFDKDAKTVTLEDNGIGMNRDDVIAHLGTIAKSGTADFLKNLSGDQKKDS
HLIGQFGVGFYSAFIVADKVDVYSRRAGTPAGEGVHWSSAGEGEFEVATVEKAERGTRIV
LHLKKGEEEFADGWRLRNIVKKYSDHIALPIELPKEFHGEEQDKSTEPEWETVNRASALW
TRPRTDVKDEEYQEFYKHVAHDFDNSLSWSHNKVEGKLEYTSLLYVPGRAPFDLYHREAP
KGLKLYVQRVFIMDQADEFLPLYLRFIKGVVDSNDLSLNVSREILQKDPVIDSMKSALTK
RVLDMLEKLAKDKPEDYKKFWSQFGQVLKEGPAEDFANKEKIASLLRFASTSDASGEQSV
SLADYLGRVKEGQDKIYYLTGESYAQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYLTD
FDGKQLADVARGDLDLGKLDSEEDKKAQEEIAKTKEGLIERLKGALGDEVAEVRVSHRLT
DSPAILAIGEQDLGLQMRQILEASGQKVPESKPIFEINPQHPLIEKLDAEPDEDRFADLS
HILFDQAALAAGDSLKDPAAYVQRLNKLLVELSA