Protein Info for Psest_2440 in Pseudomonas stutzeri RCH2

Updated annotation (from data): isobutyryl-CoA dehydrogenase (EC 1.3.8.5)
Rationale: Important for valine utilization as N source, and isobutyryl-CoA is an intermediate in valine catabolism. SEED annotated it as butyryl-CoA dehydrogenase, which is also expected to perform this reaction
Original annotation: Acyl-CoA dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 7 to 118 (112 residues), 135.5 bits, E=2e-43
• PF02770: Acyl-CoA_dh_M
  • amino acids 123 to 218 (96 residues), 92 bits, E=4.2e-30
• PF00441: Acyl-CoA_dh_1
  • amino acids 230 to 378 (149 residues), 179.6 bits, E=8.6e-57
• PF08028: Acyl-CoA_dh_2
  • amino acids 245 to 367 (123 residues), 106.1 bits, E=3.5e-34

Best Hits

Swiss-Prot: 51% identical to ACDB_BACSU: Acyl-CoA dehydrogenase (mmgC) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 98% identity to psa:PST_1922)

MetaCyc: 58% identical to short-chain acyl-CoA dehydrogenase monomer (Pseudomonas putida KT2440)
RXN-13449 [EC: 1.3.8.1]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• oleate β-oxidation (30/35 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• L-isoleucine degradation I (5/6 steps found)
• valproate β-oxidation (7/9 steps found)
• L-valine degradation I (6/8 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.1, 1.3.8.5, 1.3.99.2

Use Curated BLAST to search for 1.3.8.1 or 1.3.8.5 or 1.3.99.2

Sequence Analysis Tools

See L0GMF3 at UniProt or InterPro

Protein Sequence (383 amino acids)

>Psest_2440 isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (Pseudomonas stutzeri RCH2)
MHDLELSEDQRMIRDMARDFARREIAPHAQAWEKAGWIDDTLVAQMGELGLLGMVVPEEW
GGSYIDYVAYALAVEEISAGDGATGALMSIHNSVGCGPVLNYGSQAQKDEWLTELASGRA
IGCFALTEPQAGSEAHNLRTRAELVDGHWVLNGSKQFCSNAKRSKLAIVFAVTDPELGKK
GLSAFLVPTDTPGFAVERSEHKMGIRASDTCGVSLSDCRIPEANLLGERGKGLAIALSNL
EGGRIGIGAQALGIARAAFEAALLYARERVQFGKPIAEHQSIANMLADMQTQLNAARLLI
LHAARLKSAGLPCLSEASQAKLFASEMAEKVCSQAVQIHGGYGYLEDYPVERYYRDARIT
QIYEGSSEIQRLLIARELANYAL