Protein Info for PGA1_c02510 in Phaeobacter inhibens DSM 17395

Annotation: S-(hydroxymethyl)glutathione dehydrogenase FrmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 TIGR02818: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase" amino acids 4 to 371 (368 residues), 612 bits, see alignment E=1.9e-188 PF08240: ADH_N" amino acids 28 to 157 (130 residues), 81 bits, see alignment E=6.8e-27 PF00107: ADH_zinc_N" amino acids 199 to 331 (133 residues), 90.7 bits, see alignment E=7.8e-30

Best Hits

Swiss-Prot: 80% identical to ADHI_RHOS4: Alcohol dehydrogenase class-3 (adhI) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: None (inferred from 91% identity to dsh:Dshi_0473)

MetaCyc: 78% identical to FlhA (Paracoccus denitrificans)
S-(hydroxymethyl)glutathione dehydrogenase. [EC: 1.1.1.284]

Predicted SEED Role

"S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 1.1.1.284)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.284

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EW01 at UniProt or InterPro

Protein Sequence (371 amino acids)

>PGA1_c02510 S-(hydroxymethyl)glutathione dehydrogenase FrmA (Phaeobacter inhibens DSM 17395)
MIRTRAAVAVAPGKPLEIMEVNLEGPKKGEVLVEIKATGLCHTDEFTRSGDDPEGIFPAI
LGHEGAGVVVEVGEGVTSLEVGDHVIPLYTPECRECEYCLNPKTNLCQAIRSTQGQGLLP
DGSTRFSMLDGTPIYHYMGCSTFANHTVVPEIALAKIRKDAPFDKVCYIGCGVTTGIGAV
INTAKVEIGSRAIVFGLGGIGLNVIQGLRLAGADQIVGVDLNPGKIEMAKQFGMTDFVNP
AEVDGDLVAHLVELTKGGADYTFDATGNVQVMRTALEAAHKGWGESIIIGVAPAGAEIST
RPFQLVTGRSWRGTAFGGAAGRTDVPKIVDWYMDGKIEIDPMITHKLSLDEINHGFDLMH
EGKSIRAVVEF