Protein Info for Psest_2413 in Pseudomonas stutzeri RCH2

Annotation: Periplasmic protein TonB, links inner and outer membranes

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details PF03544: TonB_C" amino acids 184 to 261 (78 residues), 46.7 bits, see alignment E=1.8e-16

Best Hits

KEGG orthology group: None (inferred from 94% identity to psa:PST_1948)

Predicted SEED Role

"Ferric siderophore transport system, periplasmic binding protein TonB" in subsystem Campylobacter Iron Metabolism or Hemin transport system or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNI3 at UniProt or InterPro

Protein Sequence (262 amino acids)

>Psest_2413 Periplasmic protein TonB, links inner and outer membranes (Pseudomonas stutzeri RCH2)
MALGLSAPPAPAVTQRQWSGAAGAALTTLGLHAGLFALLLTSWTPTMDAPVATQVMQTQL
ISLPPPVPVLPEPPVAEPVEAPPPPVQVEAPPQVEQADLAFKRAEREREAEQQRKQQLER
RREEQRRRDEQQRREQERLAEQARLDAQRRQAEATARAEAAERARQAAAAEAASRQYLPI
AKKPPVYPQRALDSGLQGACTVSYTVDVQGRVRSPKVVGDCHPLFIRPSLIAAQSFRYQP
RIVDGRAVEVANVQNTFHYRIE