Protein Info for Psest_2406 in Pseudomonas stutzeri RCH2

Annotation: nitrite reductase [NAD(P)H], large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 852 PF07992: Pyr_redox_2" amino acids 12 to 292 (281 residues), 171 bits, see alignment E=1.3e-53 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 13 to 806 (794 residues), 1060.7 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 154 to 224 (71 residues), 53.7 bits, see alignment E=7.5e-18 PF18267: Rubredoxin_C" amino acids 326 to 394 (69 residues), 35 bits, see alignment E=3.4e-12 PF04324: Fer2_BFD" amino acids 427 to 470 (44 residues), 32 bits, see alignment 3.8e-11 PF03460: NIR_SIR_ferr" amino acids 565 to 627 (63 residues), 43.9 bits, see alignment 4.9e-15 PF01077: NIR_SIR" amino acids 637 to 758 (122 residues), 82.1 bits, see alignment E=9.1e-27

Best Hits

Swiss-Prot: 62% identical to NIRB_KLEOX: Nitrite reductase [NAD(P)H] large subunit (nasB) from Klebsiella oxytoca

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 93% identity to psa:PST_1955)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJJ3 at UniProt or InterPro

Protein Sequence (852 amino acids)

>Psest_2406 nitrite reductase [NAD(P)H], large subunit (Pseudomonas stutzeri RCH2)
MSSTVTPLTLEKLVIVGNGMVGHHCIEQLIERGALTRYELHVFGEERQRAYDRVHLSEYF
GGRDAESLAMCEADYYASHGVYTHLGVQVLGIDRERKEVITSAGRQPYDQLILATGSYPF
VPPITGAEGSARLVYRTLDDLDAIRAAATNARRGVVVGGGLLGLEAANALKSLGLEAHVV
EFAPRLMPVQLDGEGGAALKARIESLGVGVHLSRATQEIVAGEDYAWRMNFTDGEFLETD
LIVFSAGIRPQDALGRVAELEIAQRGGIVIDSECRTSDPAIFAIGECASWNGSVFGLVAP
GYSMARSVAAQLAEEPHEPFYGADMSTKLKLLGVDVGSIGDAHGATPGSRSYRFIDEASA
SYRRLVVSADGKTVLGAVLVGDNSYYDTLLQYAQNGIKLPADPSGLILPQTEGAPTLGPD
ALPASATICSCHNVSKGAVCCQVDAGVTDLGELKAATKAATGCGGCNALLKLVFDAELAA
RGVAVDKSLCEHFAYTRQELYGIVRVEGIRSFAELLAKHGRGHFGCDICKPTVGSILASC
WNQPITDPALIPLQDTNDTFMANMQKNGTYSVVPRIPGGEITPEGLIAIGQVAKKYDLYT
KITGGQRIDLFGAQLHELPDIWGELIAAGFETGHAYGKSLRTVKSCVGSTWCRYGVQDSV
GMALRLEDRYKGLRAPHKIKFAVSGCTRECAEAQSKDIGVIATDKGWNLYVCGNGGMRPR
HAELFATDLDDEALIRTIDRVLMFYMRTADKLQRTSVWRESLEGGLDYLKAVVLDDSLGL
AAELESQMQHVVDSYECEWANAINDPEKLKRFRTFVNDRRGDPDIHFVKERGQRRPVRAS
ELHLIPLTQEVL