Protein Info for PGA1_c02470 in Phaeobacter inhibens DSM 17395

Annotation: putative serine-protein kinase, PrkA type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 PF08298: AAA_PrkA" amino acids 20 to 381 (362 residues), 595.6 bits, see alignment E=3.3e-183 PF06798: PrkA" amino acids 383 to 636 (254 residues), 247.7 bits, see alignment E=1.6e-77

Best Hits

Swiss-Prot: 66% identical to YEAG_ECO57: Uncharacterized protein YeaG (yeaG) from Escherichia coli O157:H7

KEGG orthology group: K07180, serine protein kinase (inferred from 94% identity to sit:TM1040_0532)

MetaCyc: 66% identical to protein kinase YeaG (Escherichia coli K-12 substr. MG1655)
2.7.11.d,2.7.11.11,2.7.12.1,2.7.11.12,2.7.11.17,2.7.11.1,2.7.11.13,2.7.11.22,2.7.11.21,2.7.11.34,2.7.11.31 [EC: 2.7.11.1, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.17, 2.7.11.21, 2.7.11.22, 2.7.11.31, 2.7.11.34, 2.7.11.d, 2.7.12.1]

Predicted SEED Role

"Serine protein kinase (prkA protein), P-loop containing"

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.11.1 or 2.7.11.11 or 2.7.11.12 or 2.7.11.13 or 2.7.11.17 or 2.7.11.21 or 2.7.11.22 or 2.7.11.31 or 2.7.11.34 or 2.7.11.d or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DLG3 at UniProt or InterPro

Protein Sequence (644 amino acids)

>PGA1_c02470 putative serine-protein kinase, PrkA type (Phaeobacter inhibens DSM 17395)
MKDIAEIFAEQYGETREQEMSLQDYLNACRDDPSMYANVAERMLKAIGDEELVDTSKDAR
LGPIFQNRTIKRYSAFKDFYGMEDTIERIVGYFRHAAQGLEERKQILYLLGPVGGGKSSL
AERLKRLVEEQPIYVLKAGDHLSPIFESPLGLFDPVRMGKLLNDEYGIAQHRLPGLMSPW
AVQRLDEFGGDINKFSVVRLYPSRLRRICVAKVEPGDENNQDISTLVGKVDIRQLEHFSQ
DNPDAYSFSGGLNRATQGVLEFVEMFKAPIKMLHPLLTATQEGNYMGTESFGAIPFTGLI
LAHSNESEWQQFKNNKNNEAFIDRVSIVKVPYCLRVSEEAKIYDKLIEHSELVAAPCAPE
TLKILSRFSVLTRLKPHENSNLYSKLRVYDGESLKDTDPKAKTVQEYQDRAGVDEGMNGI
STRFAFKVLSTTFNFDTDEVAADPVHLMYVLEQMIKREQFPAETEAKYLAFIKTELAPRY
EEFIGNEIQKAYLESYTEYGQNVFDRYISYADAWIEEQDYKDPDTGQLYDREVLDAELSK
IEKPVGIANPKDFRNEVVKFALRHRANTGSNPAWNSYEKLRDVIEKHMFSQVEELLPVIS
FGSKKDSKTEGKHQEFVERMRSHGYTDRQVRRLVEWYMRVNKAG