Protein Info for PGA1_c23610 in Phaeobacter inhibens DSM 17395

Annotation: phosphoribosylamine--glycine ligase PurD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00877: phosphoribosylamine--glycine ligase
  • amino acids 1 to 420 (420 residues), 533.5 bits, E=1.7e-164
• PF02844: GARS_N
  • amino acids 1 to 100 (100 residues), 127.6 bits, E=7.2e-41
• PF01071: GARS_A
  • amino acids 101 to 292 (192 residues), 259.6 bits, E=5e-81
• PF02655: ATP-grasp_3
  • amino acids 107 to 287 (181 residues), 25.5 bits, E=3.2e-09
• PF02843: GARS_C
  • amino acids 327 to 418 (92 residues), 112.5 bits, E=2.4e-36

Best Hits

Swiss-Prot: 58% identical to PUR2_BRUSU: Phosphoribosylamine--glycine ligase (purD) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 89% identity to sit:TM1040_1946)

MetaCyc: 54% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (5/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Sequence Analysis Tools

See I7DSK1 at UniProt or InterPro

Protein Sequence (421 amino acids)

>PGA1_c23610 phosphoribosylamine--glycine ligase PurD (Phaeobacter inhibens DSM 17395)
MNILILGSGGREHALAWAVMQNPKCDKLIVAPGNAGIAQIADCASLDIDNGGAVAAFAEE
NAIDFVIIGPEAPLAAGVADRLRDAGLLVFGPSEAAAKLEASKSFTKEICDAAKAPTAAY
GHFTDAEAAKAHVRNHGAPTVVKADGLAAGKGVIIAMTEAEAIEAIDDMFGGAFGGAGAE
VVIEEFMEGEEASLFVLVDGEDVLAIGSAQDHKRVGEGDTGPNTGGMGAYSPAPVLSAEI
EAKAMEEIVKPTMRVMAERGMPYQGVLYAGLMIKDGQPRLVEYNVRFGDPECQVLMMRLG
AQALDLMQAAAEGRLAETQVNWADDHAITVVMAAEGYPGSYKKGSEIKGLEALPEDSSNM
VFHAGTKAEAGKMQAVGGRVLNVTARGDTLQEARDRAYAMVDGVDWPQGFFRRDIGWRAL
K