Protein Info for PGA1_c23300 in Phaeobacter inhibens DSM 17395

Annotation: putative sarcosine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 815 PF01266: DAO" amino acids 10 to 365 (356 residues), 243.3 bits, see alignment E=1.9e-75 PF16350: FAO_M" amino acids 368 to 423 (56 residues), 71.8 bits, see alignment 1.6e-23 PF01571: GCV_T" amino acids 425 to 699 (275 residues), 264.6 bits, see alignment E=2.8e-82 PF08669: GCV_T_C" amino acids 724 to 807 (84 residues), 62.8 bits, see alignment E=8e-21

Best Hits

KEGG orthology group: None (inferred from 79% identity to rec:RHECIAT_CH0000092)

Predicted SEED Role

"Sarcosine dehydrogenase (EC 1.5.99.1)" (EC 1.5.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.99.1

Use Curated BLAST to search for 1.5.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E2N1 at UniProt or InterPro

Protein Sequence (815 amino acids)

>PGA1_c23300 putative sarcosine dehydrogenase (Phaeobacter inhibens DSM 17395)
MTELPSTAKAVIIGGGIIGCSTAYHLAKLGWTDTVLLERKKLTSGTTFHAAGLVGQLRSN
ANITQLLGYSVDLYNKIETETGLGTGWKMNGGLRLACNEERWTEVKRQATTAHSFGLEME
LLTPKEAQDLWPLMDISDVIGAAFMPTDGQANPSDITQALAKGARMAGAKIFEDTKVIDI
EIEDGRIRAVITEHGRIECEKVICCAGQWTRTFAKRFGVNVPLVPMEHQYMVTEPFEGVP
SNLPTLRDPDRLTYYKEEVGGLVMGGYEPNPIPWATDGIPKGFHYSLLDSNFDHFEQLME
QALGRVPALEHAGIKTLTNGPESFTPDGNFIIGEAPELSNFYVGAGFNAFGIAAGGGAGM
ALAEWVKNGEPPFDLWSADIRRFGRPHFDTDWIRTRTVEAYGKHYTMAWPHEEHDSGRPC
RKSPLYDTLKNKGACFGEKLGWERPNWFADATRGETPQDLYSFGRQNWFDAVGREHKAAR
EAAVLFDQTSFAKFTLKGPDALAAMNWICANDVDKPVGSLIYTQMLNDKGGIECDLTVGR
VAQDEFYIVTGTGYVTHDFDWIRRNIPEGMNCQLFDITSSNAVLSLMGPKARDILAAVTR
DDVSNDGFQFGTIRTIGIAGCPVQALRVTYVGELGWELHLPVEYAQTVYAALMGVGQPLG
LVDAGYRAIESLRLEKGYRAWGSDIGPDHTPFEAGLGWAVKLRKKIAFKGRAAAEARKAG
GVKKMLACFTTDPGVVLMGRETIYRNGKRVGWLTSGGYGYTVGQSIGYGYIRNPDGVDPD
YVLSGDYELEVATQRVPCNVQLGPLYDPSMSRVKV