Protein Info for Psest_2325 in Pseudomonas stutzeri RCH2

Annotation: alpha-L-glutamate ligase-related protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 327 TIGR02291: alpha-L-glutamate ligase homolog" amino acids 6 to 324 (319 residues), 564.7 bits, see alignment E=2.4e-174 PF14397: ATPgrasp_ST" amino acids 21 to 294 (274 residues), 333 bits, see alignment E=3.3e-103 PF08443: RimK" amino acids 238 to 278 (41 residues), 23.1 bits, see alignment 1.3e-08

Best Hits

KEGG orthology group: None (inferred from 98% identity to psa:PST_2030)

Predicted SEED Role

"FIG002781: Alpha-L-glutamate ligase family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLH8 at UniProt or InterPro

Protein Sequence (327 amino acids)

>Psest_2325 alpha-L-glutamate ligase-related protein (Pseudomonas stutzeri RCH2)
MFGLIKTWKALEAKGIMGINRRNADYVLKYNKRNLYPIVDDKIITKQRAIEAGIHVPEMY
GIIETEKDIDKLKDIVKDHTDFVVKPAQGAGGDGILVIADRFEGRYKTVSGKIMSHDEIE
HQISNILTGLYSLGGHRDRALIEYRVTPDPIFKSISYEGVPDIRIIVLMGYPIMAMLRLP
TRQSGGKANLHQGAIGVGVDLATGITLRGTWLNNKITKHPDTTNAVDGVQLPNWDGFMKL
AAGCYELCGLGYIGVDMVLDQDKGPLILELNARPGLNIQIANDCGLTHRAHAVENRIAEL
KEKGIQETPEERVKFSQELFGHVPPHA