Protein Info for Psest_2301 in Pseudomonas stutzeri RCH2

Annotation: 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 121 (116 residues), 147.9 bits, E=1.4e-47
• PF02882: THF_DHG_CYH_C
  • amino acids 124 to 281 (158 residues), 222.1 bits, E=2.5e-70

Best Hits

Swiss-Prot: 98% identical to FOLD_PSEU5: Bifunctional protein FolD (folD) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 98% identity to psa:PST_2054)

MetaCyc: 67% identical to bifunctional methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase (Escherichia coli K-12 substr. MG1655)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• folate transformations I (9/13 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• L-histidine degradation III (2/6 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See L0GM10 at UniProt or InterPro

Protein Sequence (284 amino acids)

>Psest_2301 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase (Pseudomonas stutzeri RCH2)
MTAKLIDGKTIAANIRQQISGRVAERRAQGLRAPGLAVILVGSDPASQVYVAHKRKDCEE
VGFNSIAHDLPSDTRQEDLLALIDQLNDDASIDGILVQLPLPKHLDASQLLERIRPDKDV
DGFHPYNVGRLAQRMPLLRPCTPKGIMTLLESTGVDLHGLDAVVVGASNIVGRPMALELL
LAGCTTTVTHRFTHNLAEHVQRADLVVVATGITGLVKGEWIKPGAIVIDVGINRQADGRL
LGDVEFGPASERAAWITPVPGGVGPMTRACLLENTLHAAEHLHG