Protein Info for Psest_2285 in Pseudomonas stutzeri RCH2

Annotation: ATP-dependent protease La

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 PF02190: LON_substr_bdg" amino acids 6 to 197 (192 residues), 194.4 bits, see alignment E=6.5e-61 TIGR00763: endopeptidase La" amino acids 8 to 766 (759 residues), 1004.5 bits, see alignment E=2.1e-306 PF00004: AAA" amino acids 348 to 485 (138 residues), 80 bits, see alignment E=6.8e-26 PF07728: AAA_5" amino acids 348 to 480 (133 residues), 34.9 bits, see alignment E=4.6e-12 PF05362: Lon_C" amino acids 565 to 767 (203 residues), 328.2 bits, see alignment E=5.6e-102 PF13541: ChlI" amino acids 655 to 735 (81 residues), 27.8 bits, see alignment E=6.2e-10

Best Hits

Swiss-Prot: 71% identical to LON_ECOL6: Lon protease (lon) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 98% identity to psa:PST_2063)

MetaCyc: 71% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNB1 at UniProt or InterPro

Protein Sequence (798 amino acids)

>Psest_2285 ATP-dependent protease La (Pseudomonas stutzeri RCH2)
MKTTIELPLLPLRDVVVYPHMVIPLFVGREKSIEALESAMAGDKQILLLAQKNPADDDPA
EDALYRVGTVATVLQLLKLPDGTVKVLVEGEQRGVIERFVEVDDHCRAEVSLIEEAEVEA
RESEVFTRSLLSQFEQYVQLGKKVPAEVLSSLNSIDEPARLVDTMAAHMALKIEQKQQIL
EITDLAARVEHVLALLDAEIDLLQVEKRIRGRVKKQMERSQREYYLNEQMKAIQKELGDI
DEGHNEVDELKKRIENAGLSKDAYAKAQAELNKLKQMSPMSAEATVVRTYIDWLVNVPWK
AASKVRLDLAKAEEVLDADHYGLEEVKDRILEYLAVQKRVKKLKGPVLCLVGPPGVGKTS
LAESIARSTNRKFVRMALGGVRDEAEIRGHRRTYIGSMPGRLIQKMTKVGVRNPLFLLDE
IDKMGSDMRGDPASALLEVLDPEQNHNFNDHYLEVDYDLSDVMFLCTANSMNIPAPLLDR
MEVIRLPGYTEDEKINIATRYLAPKQIEANGLKKTELEFQEAAIRDIIRYYTREAGVRSL
ERQLAKVCRKVVKEHAAEKRFHVVVDADSLEHFLGIRKFRYGLAEQQDQVGQVTGLAWTQ
VGGELLTIEAAVVPGKGRLTRTGSLGEVMGESITAALTVVRSRATSLGIAADFHEKQDIH
IHVPEGATPKDGPSAGIGMCTALVSALTQIPVRADVAMTGEITLRGQVLAIGGLKEKLLA
AHRGGIKTVIIPEENQRDLKEIPENIKQDLQIKPVKWIDEVLQIALQYAPEPLPDAAPEI
VAKDDKREPDSKERISTH