Protein Info for Psest_2273 in Pseudomonas stutzeri RCH2

Annotation: coenzyme PQQ biosynthesis protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 TIGR02108: coenzyme PQQ biosynthesis protein B" amino acids 3 to 303 (301 residues), 446.7 bits, see alignment E=1.8e-138 PF12706: Lactamase_B_2" amino acids 51 to 270 (220 residues), 166.9 bits, see alignment E=2.3e-53

Best Hits

Swiss-Prot: 96% identical to PQQB_PSEU5: Coenzyme PQQ synthesis protein B (pqqB) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K06136, pyrroloquinoline quinone biosynthesis protein B (inferred from 96% identity to psa:PST_2073)

Predicted SEED Role

"Coenzyme PQQ synthesis protein B" in subsystem Coenzyme PQQ synthesis or Pyrroloquinoline Quinone biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLY4 at UniProt or InterPro

Protein Sequence (303 amino acids)

>Psest_2273 coenzyme PQQ biosynthesis protein B (Pseudomonas stutzeri RCH2)
MFIRILGSAAGGGFPQWNCNCANCAGLRAGTLNAKARTQSSIAISDNGVDWVLCNASPDI
RAQLESFPALQPARAPRDTAIGAIVLLDSQIDHTTGLLTLREGCPHEVWCTEMVHQDLTT
GFPLFNMLEHWNGGLKWNRIGLTDSFSIPCCPNLHITPIPLRSSAPPYSPHRNDPHPGDN
IGLLIEDRKSGGTLFYAPGLGKVSNELLETMRQADCLLVDGTLWRDDEMLVREVGTKLGS
EMGHLQQSGPGGMIEVLDGMPAVRKILIHINNTNPILDEDSVEREILGEHGIEVAFDGMN
IEL