Protein Info for Psest_2270 in Pseudomonas stutzeri RCH2

Annotation: coenzyme PQQ biosynthesis protein E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 TIGR02109: coenzyme PQQ biosynthesis enzyme PqqE" amino acids 16 to 373 (358 residues), 611.6 bits, see alignment E=4.1e-188 PF04055: Radical_SAM" amino acids 25 to 181 (157 residues), 99.9 bits, see alignment E=2.8e-32 PF13353: Fer4_12" amino acids 28 to 109 (82 residues), 23.6 bits, see alignment E=8.3e-09 PF13186: SPASM" amino acids 252 to 317 (66 residues), 37.7 bits, see alignment E=3.2e-13 TIGR04085: radical SAM additional 4Fe4S-binding SPASM domain" amino acids 260 to 344 (85 residues), 30.6 bits, see alignment E=3.5e-11

Best Hits

Swiss-Prot: 87% identical to PQQE_PSEMY: PqqA peptide cyclase (pqqE) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K06139, pyrroloquinoline quinone biosynthesis protein E (inferred from 87% identity to pmy:Pmen_1969)

MetaCyc: 73% identical to glutamate Cgamma--tyrosine C3 ligase (Klebsiella pneumoniae)
RXN-11176 [EC: 1.21.98.4]

Predicted SEED Role

"Coenzyme PQQ synthesis protein E" in subsystem Coenzyme PQQ synthesis or Pyrroloquinoline Quinone biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.21.98.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNA0 at UniProt or InterPro

Protein Sequence (384 amino acids)

>Psest_2270 coenzyme PQQ biosynthesis protein E (Pseudomonas stutzeri RCH2)
MNGSGSSFAKPGYEPGPPLWLLAELTYRCPLQCPYCSNPLDFARSHDELSTAEWIEVFRQ
AREMGAAQLGFSGGEPLVRQDLVELIEAARGLGYYTNLITSGIGLTEEKIASFAEAGLDH
IQISFQAADEEVNNLLAGSKKAFAHKLEMARAVKKHGYPMVLNFVTHRHNIDNIEQIIRL
CIELEADFVELATCQFYGWAELNRAGLLPSKEQLVRAERITNEWRDKLAAENHPCKLIFV
TPDYYEERPKACMNGWGNLFLDITPDGTALPCHSARQLPVEFPNVREHSIEHIWRHSFGF
NKFRGYDWMPEPCRSCDEKERDFGGCRCQAFMLTGDAANADPVCSKSLHHDKILAARAQA
EHGALSLEQLQHRNEKASKLILKV