Protein Info for Psest_2268 in Pseudomonas stutzeri RCH2

Annotation: Alcohol dehydrogenase, class IV

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 PF00465: Fe-ADH" amino acids 14 to 379 (366 residues), 388.6 bits, see alignment E=2.6e-120 PF13685: Fe-ADH_2" amino acids 19 to 112 (94 residues), 33.1 bits, see alignment E=5.3e-12

Best Hits

Swiss-Prot: 40% identical to DHAT_CITFR: 1,3-propanediol dehydrogenase (dhaT) from Citrobacter freundii

KEGG orthology group: None (inferred from 97% identity to psa:PST_2078)

MetaCyc: 40% identical to alcohol dehydrogenase II monomer (Zymomonas mobilis)
Alcohol dehydrogenase. [EC: 1.1.1.1]

Predicted SEED Role

"Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10)" (EC 1.1.1.1, EC 1.2.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1

Use Curated BLAST to search for 1.1.1.1 or 1.2.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLX8 at UniProt or InterPro

Protein Sequence (387 amino acids)

>Psest_2268 Alcohol dehydrogenase, class IV (Pseudomonas stutzeri RCH2)
MSPNISLQRKFVSPEIIFGAGCRHSVGNCAANFGARKVLLVSDPGVVHAGWVADVQASLE
RQNIGHCLFTGVSPNPRCEEVMLGAELYRSEGCNVIVAVGGGSPMDCAKGIGIVAAHGRH
IYEFEGVDTLRVPSPPLILIPTTAGTSADVSQFVIISNQQERMKFSIVSKAAVPDVSLID
PETTLSMDPFLSACTGIDALVHAIEAFVSTGHGPLTDPHALEAMRLINGNLVQMIANPAD
IALREQIMLGSMQAGLAFSNAILGAVHAMSHSLGGYLDLPHGVCNAVLVEHVVAFNYDAA
PDRYRMVAETLGIDSRGLSHRQVRERLVQHLIDLKQQIGFRETLGLHGVNLSDIPFLSQH
AMQDPCILTNPRSSTQRDVEVVYAEAL