Protein Info for Psest_2259 in Pseudomonas stutzeri RCH2

Annotation: excinuclease ABC, C subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 607 TIGR00194: excinuclease ABC subunit C" amino acids 11 to 585 (575 residues), 612.9 bits, see alignment E=3.3e-188 PF01541: GIY-YIG" amino acids 18 to 93 (76 residues), 40.8 bits, see alignment E=5.5e-14 PF02151: UVR" amino acids 205 to 232 (28 residues), 31.6 bits, see alignment (E = 2.5e-11) PF08459: UvrC_RNaseH_dom" amino acids 383 to 538 (156 residues), 184.7 bits, see alignment E=2.8e-58 PF14520: HHH_5" amino acids 553 to 604 (52 residues), 47.4 bits, see alignment 5.6e-16

Best Hits

Swiss-Prot: 85% identical to UVRC_PSEF5: UvrABC system protein C (uvrC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03703, excinuclease ABC subunit C (inferred from 98% identity to psa:PST_2086)

MetaCyc: 52% identical to UvrABC excision nuclease subunit C (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit C" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJ60 at UniProt or InterPro

Protein Sequence (607 amino acids)

>Psest_2259 excinuclease ABC, C subunit (Pseudomonas stutzeri RCH2)
MTETFDSSAFLAACSGRPGVYRMFDAQAKLLYVGKAKNLKKRLASYFRKTGQAPKTAALV
AKIAQVETTITANETEALLLEQTLIKQWRPPYNILLRDDKSYPYVFLSAGEFPRLSIHRG
AKKEPGRYFGPYPSAGAIRESLSLLQKAFFVRQCEDSYYRNRTRPCLQYQIKRCKAPCVN
LVEPAEYAEDVRHSVMFLEGRSNALAEELSRNMEKAAMSLDFERAAEIRDQIGILRRVQD
QQSMEGGTGDVDIVAAVVSPGGACVHLITVRGGRVLGSKNFFPQVAIEESVSEVLQAFLE
QYYLGAAERDLPAELIVNAVHEDFATLIDAISELRGVELTITHRVRGTRARWQQLALTNA
EQAMGARLANRQHLSARFEALAEALELDEPPQRLECFDISHSSGEATVASCVVFGPEGPL
KSDYRRYNIEGVTAGDDYAAMHQALSRRFRKAAEGEGKLPDILLVDGGKGQLNMAREVLE
ELAVPELILLGVAKGVTRKPGLETLYLNDAAHEFTLPADSPALHLIQQVRDEAHRFAITG
HRARRGKARRTSTLEEVPGIGPKRRRELLTHFGGLQELCRASLDEIAKAPGISKKLAESI
YAALHSE