Protein Info for Psest_0221 in Pseudomonas stutzeri RCH2

Annotation: phosphate ABC transporter, permease protein PstA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 306 to 333 (28 residues), see Phobius details amino acids 353 to 374 (22 residues), see Phobius details amino acids 396 to 416 (21 residues), see Phobius details amino acids 444 to 465 (22 residues), see Phobius details amino acids 521 to 543 (23 residues), see Phobius details TIGR00974: phosphate ABC transporter, permease protein PstA" amino acids 288 to 548 (261 residues), 249.3 bits, see alignment E=2e-78 PF00528: BPD_transp_1" amino acids 323 to 548 (226 residues), 64.7 bits, see alignment E=4.7e-22

Best Hits

KEGG orthology group: K02038, phosphate transport system permease protein (inferred from 97% identity to psa:PST_4025)

Predicted SEED Role

"Phosphate transport system permease protein PstA (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGE2 at UniProt or InterPro

Protein Sequence (556 amino acids)

>Psest_0221 phosphate ABC transporter, permease protein PstA (Pseudomonas stutzeri RCH2)
MKKDSLNSWVKSGTPWIWMNAGAVSIAVIMTLGLLAIIAVRGLAHFWPADVIVADYSMPG
AEKRVLAGEVVQAEEVPRARLAASGLPVNVEGGEFMTRELLKVGNREVYGADFSWVVGEW
LSNQHTPAELMVLERREWGNFYGYLLNVKEAGQLVAEGDSAWNELQTRIDRVDELHSRIS
RIEKVEIGRINHGLERLRLKTRQLELNDRLDAAAQADLDAERAQWDAEYRVLEDQLIALQ
QEFNRDSITVRTADGREQEITLGKVVRAFRPNAMSTPQKLMFYFAKLWEFVSDEPREANT
EGGVFPAIFGTVLMTMIMAVIVTPFGVIAAVYLREYAKQGLLTRIIRIAVNNLAGVPSIV
YGVFGLGFFVYVLGGSLDRLFYPEAAPAPVFGTPGLMWASLTLAILTLPVVIVATEEGLA
RIPRMIREGSLALGATKSETLWKVVLPMASPAMMTGLILAVARAAGEVAPLMLVGVVKLA
PNLPLNGNYPYLHLDQKIMHLGFHIYDVGFQSPNVEAARPLVYATALLLVLVIATLNFSA
IYIRNHLREKYKALDH