Protein Info for Psest_2243 in Pseudomonas stutzeri RCH2

Annotation: glyceraldehyde-3-phosphate dehydrogenase, type I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00044: Gp_dh_N
  • amino acids 3 to 101 (99 residues), 102.4 bits, E=1.4e-33
• TIGR01534: glyceraldehyde-3-phosphate dehydrogenase, type I
  • amino acids 4 to 324 (321 residues), 390.8 bits, E=2.7e-121
• PF02800: Gp_dh_C
  • amino acids 157 to 313 (157 residues), 205.3 bits, E=4.4e-65

Best Hits

Swiss-Prot: 69% identical to G3P3_NOSS1: Glyceraldehyde-3-phosphate dehydrogenase 3 (gap3) from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

KEGG orthology group: K00134, glyceraldehyde 3-phosphate dehydrogenase [EC: 1.2.1.12] (inferred from 95% identity to psa:PST_2100)

MetaCyc: 90% identical to glyceraldehyde-3-phosphate dehydrogenase (arsenate-transferring) (Pseudomonas aeruginosa)
RXN1YI0-11 [EC: 1.2.1.107]

Predicted SEED Role

"NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Pyridoxin (Vitamin B6) Biosynthesis or Redox-dependent regulation of nucleus processes (EC 1.2.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• Bifidobacterium shunt (15/15 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• homolactic fermentation (11/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• heterolactic fermentation (15/18 steps found)
• pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• Entner-Doudoroff pathway I (8/9 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• glycolysis IV (8/10 steps found)
• arsenate detoxification V (2/2 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• gluconeogenesis III (8/12 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (8/12 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• glycerol degradation to butanol (10/16 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.12

Use Curated BLAST to search for 1.2.1.107 or 1.2.1.12

Sequence Analysis Tools

See L0GN75 at UniProt or InterPro

Protein Sequence (334 amino acids)

>Psest_2243 glyceraldehyde-3-phosphate dehydrogenase, type I (Pseudomonas stutzeri RCH2)
MSIKVGINGFGRIGRLALRAAWDWPELDFVQINDPAGDAATHAHLLNFDSIHGRWKHEAG
SDGDCLVIDGQRVKVSANKAISDTDWSGCDLVIEASGKMKTVAVLQQYLEQGVKRVVVSA
PVKESGALNIVMGVNDSLFDPAQHRIVTAASCTTNCLAPVVKVIHEQLGIRHGSITTIHD
LTNTQSILDQPHKDLRRARASGMSLIPTTTGSATAIAEIFPELRGKLNGHAVRVPLANAS
LTDCVFEVERATDAAEVNRLLKAAAEGELKGILGYEERPLVSIDYRTDPRSSIIDALSTM
VVNGTQVKIYAWYDNEWGYANRTVELARNVGLAG