Protein Info for PGA1_c22110 in Phaeobacter inhibens DSM 17395

Annotation: putative glutathione-binding protein GsiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 signal peptide" amino acids 1 to 47 (47 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 18 to 46 (29 residues), 17.5 bits, see alignment (E = 2e-07) PF00496: SBP_bac_5" amino acids 98 to 447 (350 residues), 267.4 bits, see alignment E=1.1e-83

Best Hits

KEGG orthology group: K02035, peptide/nickel transport system substrate-binding protein (inferred from 74% identity to sil:SPO2995)

Predicted SEED Role

"Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) or Sex pheromones in Enterococcus faecalis and other Firmicutes (TC 3.A.1.5.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EYE2 at UniProt or InterPro

Protein Sequence (528 amino acids)

>PGA1_c22110 putative glutathione-binding protein GsiB (Phaeobacter inhibens DSM 17395)
MKDQLDFMTQSVTSGKMTRREFMGKTAALGISAAVAGSMFARAAEAAGPVKGGTLKLGSI
GGGSTDSLDPAVAASQVPYHNLYQFGENLVNVTPEGGIENRVAESVEASADAKTWTFKIR
KGVEFHNGKPVTAEDVMRTMERHSNEDSKSGALGIMRGIESMKADGDNFIVELTTPNADL
PYLMADYHLMIQPDGGFDNPAAGIGSGAYMLEADEPGVRHMWKKNPNYWDDTRGHVDEVE
IVVINDATARMAALQSGQVHIVNRVEPKVAGLLDRAPNLTVRNAAGPGHYVFIMHCDTAP
FDNNELRLALKYAINREEMVDKVLRGYGSVGNDMPVNAAYPLFDDSIPQRPFDPAKAAEH
YKKSGHDGSPITLRVSDVAFPGALDAAQLFQQSANAAGIPLELKREPGDGYWSEVWNAQP
FCASYWGGRPVQDQMYSTAYLSTADWNDTRFKREDFDKLLFAARGELDEAKRKALYSQMG
MMVRDEGGLICPMFNDFIEATSSKVQGWVVDSTGDTMAGKWSHKCWLA