Protein Info for HP15_2099 in Marinobacter adhaerens HP15

Annotation: DEAD/DEAH box helicase domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 PF00270: DEAD" amino acids 35 to 210 (176 residues), 162 bits, see alignment E=1.7e-51 PF04851: ResIII" amino acids 54 to 206 (153 residues), 35.4 bits, see alignment E=1.5e-12 PF00271: Helicase_C" amino acids 247 to 355 (109 residues), 104.1 bits, see alignment E=7.6e-34

Best Hits

Swiss-Prot: 56% identical to RHLB_PSEPK: ATP-dependent RNA helicase RhlB (rhlB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03732, ATP-dependent RNA helicase RhlB [EC: 3.6.4.13] (inferred from 91% identity to maq:Maqu_1776)

MetaCyc: 45% identical to ATP-dependent RNA helicase RhlB (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase RhlB" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PRF4 at UniProt or InterPro

Protein Sequence (427 amino acids)

>HP15_2099 DEAD/DEAH box helicase domain protein (Marinobacter adhaerens HP15)
MTSDPKHTGDLRFSDLNLDKRLLDAITAIGFEYCTPIQAETLPWTLACEDLIGQAQTGTG
KTAAFLITAIQSLLETPIPEKERFASEPRVLALAPTRELAMQIAKDAEQLCQHTGHNVVT
VVGGMNYDKQRDQLQNEIVDILVATPGRLIDFLGSQDVFLDQLDILILDEADRMLDMGFI
PDVKRIIRKCTPKEERQTLLFSATFNQDVLNLASMWTSNAEFVEIEPEQKTAERVEQTVY
LVGDDEKLPVLVNFLKRPEVEKALVFANRRDQCRDLEEDLRNQGVKVALMSGEIAQNKRL
KTLDQFKKGSIQVLVATDVAGRGIHVNGVTHVFNYNLPDNAEDYVHRIGRTGRAGKHGVS
ISFAGEDDSFALPAIETYISQKLKTAVPDEALMAPMDNPPITRKRGRRPQGGGGRGQGGR
NQRQRRN