Protein Info for PGA1_c21680 in Phaeobacter inhibens DSM 17395

Annotation: choline-sulfatase BetC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 TIGR03417: choline-sulfatase" amino acids 3 to 499 (497 residues), 892.3 bits, see alignment E=5e-273 PF00884: Sulfatase" amino acids 4 to 345 (342 residues), 138.4 bits, see alignment E=6.5e-44 PF01663: Phosphodiest" amino acids 7 to 291 (285 residues), 35.8 bits, see alignment E=1.5e-12 PF16347: SGSH_C" amino acids 295 to 434 (140 residues), 44.1 bits, see alignment E=5.4e-15 PF12411: Choline_sulf_C" amino acids 447 to 498 (52 residues), 72.2 bits, see alignment 4.7e-24

Best Hits

Swiss-Prot: 72% identical to BETC_RHIME: Choline-sulfatase (betC) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01133, choline-sulfatase [EC: 3.1.6.6] (inferred from 87% identity to sil:SPO1083)

MetaCyc: 72% identical to choline sulfatase (Sinorhizobium meliloti Rm2011)
Choline-sulfatase. [EC: 3.1.6.6]

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.6

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E2A9 at UniProt or InterPro

Protein Sequence (502 amino acids)

>PGA1_c21680 choline-sulfatase BetC (Phaeobacter inhibens DSM 17395)
MTAPNILIVMVDQLNGTLFPDGPADFLHAPNLKKLAARSTRFKNAYTASPLCAPGRASFM
SGQLPSRTGVYDNAAEFRSDIPTYAHHLRRAGYYTCLSGKMHFVGPDQMHGFEDRLTTDI
YPADFGWTPDYRKPGERIDWWYHNMGSVTGAGIAEISNQMEYDDEVAYHAKAKLYDLARG
KDDRPWALTVSFTHPHDPYVARRKYWDLYEDCEHLLPEVPAMDYADHDNHSKRIFDANDW
RSFDISEEDIKRSRRAYFANISYLDDKIGEILEVLETTRQEAIILFVSDHGDMLGERGLW
FKMSFYEGSSRVPLMISAPNLPAGLIETPVSTLDVTPTLGALAGVDMAEIEPWTDGQNLL
PLALGTERSEPVAIEYAAEASYAPLVSLRYGKWKYNRCALDPDQLFDLEADPQELNNLAG
DPAHAGTLQTLRAKSEARWDLDRFDAEVRASQARRWVVYEALRQGGYYPWDYQPLQKASE
RYMRNHMDLNDVEDGNRFPRGE