Protein Info for PGA1_c21660 in Phaeobacter inhibens DSM 17395

Annotation: choline dehydrogenase BetA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 551 PF00890: FAD_binding_2" amino acids 4 to 35 (32 residues), 23.8 bits, see alignment (E = 6.4e-09) TIGR01810: choline dehydrogenase" amino acids 4 to 532 (529 residues), 831.4 bits, see alignment E=1.6e-254 PF01266: DAO" amino acids 4 to 34 (31 residues), 28.7 bits, see alignment (E = 2.5e-10) PF00732: GMC_oxred_N" amino acids 4 to 295 (292 residues), 207.6 bits, see alignment E=5.4e-65 PF13450: NAD_binding_8" amino acids 7 to 34 (28 residues), 27 bits, see alignment (E = 1.1e-09) PF05199: GMC_oxred_C" amino acids 387 to 524 (138 residues), 141.6 bits, see alignment E=6.4e-45

Best Hits

Swiss-Prot: 71% identical to BETA_BRUO2: Oxygen-dependent choline dehydrogenase (betA) from Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 94% identity to sit:TM1040_1882)

MetaCyc: 67% identical to choline dehydrogenase (Sinorhizobium meliloti Rm2011)
Choline dehydrogenase. [EC: 1.1.99.1]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.1

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EYA1 at UniProt or InterPro

Protein Sequence (551 amino acids)

>PGA1_c21660 choline dehydrogenase BetA (Phaeobacter inhibens DSM 17395)
MNADYVIVGAGSAGCAMAYRLSEAGKSVLVIEHGGTDAGPFIQMPGALSYPMNMSMYDWG
YKSQPEPHLGGRELVCPRGKVVGGSSSINGMVYVRGHAGDYNHWAESGAAGWSYADVLPY
FKRMETWDDRGHGGDPDWRGTDGPLHVTRGPRDNPLHAAFVKAGEQAGYPVSKDYNGEQQ
EGFGPMEMTVYKGQRWSAANAYLKPALKRDNCEMIRALARKVVIEDGRAVGVEVERGGKI
EVIRANAEVILAASSLNSPKLLMLSGIGPAKHLAEHGIDVVVDRPGVGQNLQDHLEFYFQ
FASKQPITLFKYWNLFGKALVGAQWLFTKTGLGASNQFESAAFIRSDKGVDYPDIQYHFL
PIAVRYDGQAAAEGHGFQAHVGPMRSDSRGEVTLASADPNDAPKILFNYMSTEKDWEDFR
KCIRLTREVFAQDAMKPFVKHEIQPGDALQSDEELNGFIREHVESAYHPCGTCKMGAVED
PMAVVDPECRVIGVEGLRVADSSIFPRITNGNLNGPSIMTGEKASDHILGRRLPSSNAEP
WFNPNWEGSQR