Protein Info for Psest_2158 in Pseudomonas stutzeri RCH2

Annotation: alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 PF22019: GlgB_N" amino acids 19 to 106 (88 residues), 64.4 bits, see alignment E=1.7e-21 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 116 to 734 (619 residues), 930.8 bits, see alignment E=1.9e-284 PF02922: CBM_48" amino acids 132 to 214 (83 residues), 79.9 bits, see alignment E=2.8e-26 PF00128: Alpha-amylase" amino acids 276 to 355 (80 residues), 32.8 bits, see alignment E=9.8e-12 PF02806: Alpha-amylase_C" amino acids 638 to 735 (98 residues), 83 bits, see alignment E=3.2e-27

Best Hits

Swiss-Prot: 73% identical to GLGB_PSEF5: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 95% identity to psa:PST_2152)

MetaCyc: 54% identical to 1,4-alpha-glucan branching enzyme (Mycobacterium tuberculosis H37Rv)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GLN6 at UniProt or InterPro

Protein Sequence (737 amino acids)

>Psest_2158 alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase (Pseudomonas stutzeri RCH2)
MTDRPVLTMPGETLMPSDTDVDALVRAEHGNPFSILGPHPDGDGVVIRAYLPNALGVEVL
DREQQLLAVMEQGQVPGFFFTRLPHQQPYLLKTRWAGGEQISEDPYSFGPQLGELDMHLF
SEGNHRQIGRVFGSQVLEVDGVQGVRFAVWAPNARRVSVVGSFNGWDGRRHPMRLRFPSG
VWELFIPRLQPGDTYKYEILGPNGIVPLKADPIALATEHPPGTASVVARPLDYQWKDQGW
MQQRTSRQSVNAPMSIYELHAGSWRRDGGDHGRLYDWHELAERLIPYVVDLGFTHIELMP
IMEHPFGGSWGYQLLSQFAPSARYGNAHDFAEFVDACHNAGIGVILDWVPAHFPTDAHGL
GEFDGTALYEYAHPFEGFHQDWDTYIYNLGRTEVHGFMLASALHWLREFHVDALRVDAVA
SMLYRDYSRKDGEWIPNRHGGRENLEAIDFLRHLNDVVATETPGALVIAEESTAFPGVSK
PTSEGGLGFSYKWNMGWMHDSLKYVQEDPINRQYHHDKMTFSMVYAYSEHFVLPISHDEV
VHGKGSLIDKMPGDRWQKFANLRAYLSFMWTHPGKKLLFMGSEFGQWREWNHDRELDWHL
LEEADHRGVQNLVRDLNRLYSQEPALHELDSDPRGFQWLIGDDRANSVFAWLRKSSTGQP
LLVIANFTPVVREAYRIGVPVEARWLEVFNSDAECYGGSNTGNGGGMLAEPIESHGEKVS
LTLTLPPLGLLVLRPQA