Protein Info for Psest_2156 in Pseudomonas stutzeri RCH2

Annotation: Glycosidases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 670 PF11896: GlgE_dom_N_S" amino acids 21 to 204 (184 residues), 190.2 bits, see alignment E=6.3e-60 PF00128: Alpha-amylase" amino acids 228 to 394 (167 residues), 32.5 bits, see alignment E=1.1e-11 PF21702: GLGE_C" amino acids 578 to 658 (81 residues), 65.1 bits, see alignment E=8.6e-22

Best Hits

Swiss-Prot: 70% identical to GLGE_PSEAE: Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (glgE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 94% identity to psa:PST_2154)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.1

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMS7 at UniProt or InterPro

Protein Sequence (670 amino acids)

>Psest_2156 Glycosidases (Pseudomonas stutzeri RCH2)
MSDAQPNATIHPRLEEALQMPRIAIESTLPVVDEGRFAAKAVIGQPVVVTSKIFADGHDQ
LAAAVCWREKGESNWRRARLKLLGNDAWQGQFTPTQVAEHEFVIEAWWDVFESYRYELSK
KHTAGVPVHLELEEGRLLLEKATARAQGENRAVLDDLSHRLSLAHSDDERVSLLLAPETA
VAMAAADPREHCSRSRVYPLDVERTLAQFASWYELFPRSETDDPSRHGTFRDVHKRLPAI
RDMGFDVLYFPPIHPIGRQHRKGPNNSLQAGPNDPGSPYAIGSEEGGHDAIHPELGTLDD
FRELVAAAREHGLEIALDFAIQCSQDHPWLKEHPGWFSWRPDGTIKYAENPPKKYQDIVN
VDFYAEDAVPDLWLALRDVVWHWVEQGVKVFRVDNPHTKPLPFWEWLIADIRERDRDVMF
LSEAFTRPGMMARLGKIGYSQSYTYFTWRNTKAELSEYLTELNEPPLRDCYRPNFFVNTP
DINPFFLQDSGRAGFLIRAALATMGSGLWGMYSGFELCESAAIPGKEEYLDSEKYQIRFR
DYHAPGNIIAEIAQLNRIRRQNPALQTHLGFKAYVAWNDNILYFGKRTPDLSNFILVAIS
LDPHNAQEAHFELPLWELGLPDDAHTQGEDLMNGHRWTWYGKTQWMRIDPAHLPFGIWRL
TAQQPDPDLK